Journal article Open Access

Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714

Kamravamanesh, Donya; Pflügl, Stefan; Nischkauer, Winfried; Limbeck, Andreas; Lackner, Maximilian; Herwig, Christoph


DataCite XML Export

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="DOI">10.5281/zenodo.3460307</identifier>
  <creators>
    <creator>
      <creatorName>Kamravamanesh, Donya</creatorName>
      <givenName>Donya</givenName>
      <familyName>Kamravamanesh</familyName>
      <affiliation>Technical University Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Pflügl, Stefan</creatorName>
      <givenName>Stefan</givenName>
      <familyName>Pflügl</familyName>
      <affiliation>Technical University Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Nischkauer, Winfried</creatorName>
      <givenName>Winfried</givenName>
      <familyName>Nischkauer</familyName>
      <affiliation>Technical University Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Limbeck, Andreas</creatorName>
      <givenName>Andreas</givenName>
      <familyName>Limbeck</familyName>
      <affiliation>Technical University Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Lackner, Maximilian</creatorName>
      <givenName>Maximilian</givenName>
      <familyName>Lackner</familyName>
      <affiliation>University of Applied Sciences Technikum Vienna</affiliation>
    </creator>
    <creator>
      <creatorName>Herwig, Christoph</creatorName>
      <givenName>Christoph</givenName>
      <familyName>Herwig</familyName>
      <affiliation>Technical University Vienna</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2017</publicationYear>
  <subjects>
    <subject>Photobioreactor Cultivations</subject>
    <subject>Cyanobacterium</subject>
    <subject>Poly-β-hydroxybutyrate</subject>
    <subject>Synechocystis sp. PCC 6714</subject>
    <subject>Nitrogen and Phosphorus Limitation</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2017-07-06</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3460307</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3460306</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/fhtw</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Poly-&amp;beta;-hydroxybutyrate (PHB) production from CO2&amp;nbsp;has the potential to reduce the production cost of this biodegradable&amp;nbsp;polyesters,&amp;nbsp; and also to make the material more sustainable compared to utilization of sugar feedstocks. In&amp;nbsp;this study the unicellular&amp;nbsp; cyanobacterium, Synechocystis sp. PCC 6714 has been identified as an unexplored potential&amp;nbsp;organism for production of PHB.&amp;nbsp; Synechocystis sp. PCC 6714 was studied under various cultivation conditions and&amp;nbsp;nutritional limitations. Combined effects of&amp;nbsp; nitrogen and phosphorus deficiency led to highest PHB accumulation&amp;nbsp;under photoautotrophic conditions. Multivariate experimental&amp;nbsp; design and quantitative bioprocess development&amp;nbsp;methodologies were used to identify the key cultivation parameters for PHB&amp;nbsp; accumulation. Biomass growth and PHB&amp;nbsp;accumulation were studied under controlled defined conditions in a lab-scale&amp;nbsp; photobioreactor. Specific growth rates&amp;nbsp;were fourfold higher in photobioreactor experiments when cultivation conditions were&amp;nbsp; controlled. After 14 days of&amp;nbsp;cultivation in nitrogen and phosphorus, limited media intracellular PHB levels reached up to 16.4% from&amp;nbsp; CO2.&amp;nbsp;The&amp;nbsp;highest volumetric production rate of PHB was 59 &amp;plusmn; 6 mg L&amp;minus;1 day&amp;minus;1. Scanning electron microscopy of isolated PHB&amp;nbsp;granules of Synechocystis sp. PCC 6714 cultivated under nitrogen and phosphorus limitations showed an average&amp;nbsp;diameter of&amp;nbsp; 0.7 &amp;mu;m. The results of this study might contribute towards a better understanding of photoautotrophic&amp;nbsp;PHB production from&amp;nbsp; cyanobacteria.&lt;/p&gt;</description>
  </descriptions>
</resource>
109
17
views
downloads
All versions This version
Views 109109
Downloads 1717
Data volume 26.4 MB26.4 MB
Unique views 7272
Unique downloads 1717

Share

Cite as