Journal article Open Access

Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714

Kamravamanesh, Donya; Pflügl, Stefan; Nischkauer, Winfried; Limbeck, Andreas; Lackner, Maximilian; Herwig, Christoph


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.3460307", 
  "container_title": "AMB Express", 
  "language": "eng", 
  "title": "Photosynthetic poly-\u03b2-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714", 
  "issued": {
    "date-parts": [
      [
        2017, 
        7, 
        6
      ]
    ]
  }, 
  "abstract": "<p>Poly-&beta;-hydroxybutyrate (PHB) production from CO2&nbsp;has the potential to reduce the production cost of this biodegradable&nbsp;polyesters,&nbsp; and also to make the material more sustainable compared to utilization of sugar feedstocks. In&nbsp;this study the unicellular&nbsp; cyanobacterium, Synechocystis sp. PCC 6714 has been identified as an unexplored potential&nbsp;organism for production of PHB.&nbsp; Synechocystis sp. PCC 6714 was studied under various cultivation conditions and&nbsp;nutritional limitations. Combined effects of&nbsp; nitrogen and phosphorus deficiency led to highest PHB accumulation&nbsp;under photoautotrophic conditions. Multivariate experimental&nbsp; design and quantitative bioprocess development&nbsp;methodologies were used to identify the key cultivation parameters for PHB&nbsp; accumulation. Biomass growth and PHB&nbsp;accumulation were studied under controlled defined conditions in a lab-scale&nbsp; photobioreactor. Specific growth rates&nbsp;were fourfold higher in photobioreactor experiments when cultivation conditions were&nbsp; controlled. After 14 days of&nbsp;cultivation in nitrogen and phosphorus, limited media intracellular PHB levels reached up to 16.4% from&nbsp; CO2.&nbsp;The&nbsp;highest volumetric production rate of PHB was 59 &plusmn; 6 mg L&minus;1 day&minus;1. Scanning electron microscopy of isolated PHB&nbsp;granules of Synechocystis sp. PCC 6714 cultivated under nitrogen and phosphorus limitations showed an average&nbsp;diameter of&nbsp; 0.7 &mu;m. The results of this study might contribute towards a better understanding of photoautotrophic&nbsp;PHB production from&nbsp; cyanobacteria.</p>", 
  "author": [
    {
      "family": "Kamravamanesh, Donya"
    }, 
    {
      "family": "Pfl\u00fcgl, Stefan"
    }, 
    {
      "family": "Nischkauer, Winfried"
    }, 
    {
      "family": "Limbeck, Andreas"
    }, 
    {
      "family": "Lackner, Maximilian"
    }, 
    {
      "family": "Herwig, Christoph"
    }
  ], 
  "type": "article-journal", 
  "issue": "7", 
  "id": "3460307"
}
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