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Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

Mann, Mandeep; Harding, Rachel; Schapira, Matthieu


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  <dc:creator>Mann, Mandeep</dc:creator>
  <dc:creator>Harding, Rachel</dc:creator>
  <dc:creator>Schapira, Matthieu</dc:creator>
  <dc:date>2019-09-10</dc:date>
  <dc:description>Expression and purification of biotinylated full length (FL) USP51-835 and biotinylated zinc finger ubiquitin-binding domain (ZnF-UBD) USP5171-290 constructs for the development of a displacement assay. </dc:description>
  <dc:description>Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dc:description>
  <dc:identifier>https://zenodo.org/record/3403804</dc:identifier>
  <dc:identifier>10.5281/zenodo.3403804</dc:identifier>
  <dc:identifier>oai:zenodo.org:3403804</dc:identifier>
  <dc:relation>doi:10.5281/zenodo.3403803</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/openlabnotebooks</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>usp5</dc:subject>
  <dc:subject>displacement assay</dc:subject>
  <dc:subject>zinc finger</dc:subject>
  <dc:subject>protein purification</dc:subject>
  <dc:title>Growth and Purification of Biotinylated USP5 Constructs &amp; Testing ZnF-UBD Ligands in a Displacement Assay</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
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