Dataset Open Access

Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

Mann, Mandeep; Harding, Rachel; Schapira, Matthieu


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{
  "description": "<p>Expression and purification of&nbsp;biotinylated full length (FL) USP5<sup>1-835</sup> and biotinylated zinc finger ubiquitin-binding domain (ZnF-UBD) USP5<sup>171-290 </sup>constructs for the development of a displacement assay.&nbsp;</p>", 
  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "University of Toronto, Structural Genomics Consortium", 
      "@id": "https://orcid.org/0000-0002-8252-6107", 
      "@type": "Person", 
      "name": "Mann, Mandeep"
    }, 
    {
      "affiliation": "University of Toronto, Structural Genomics Consortium", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "University of Toronto, Structural Genomics Consortium", 
      "@type": "Person", 
      "name": "Schapira, Matthieu"
    }
  ], 
  "url": "https://zenodo.org/record/3403804", 
  "datePublished": "2019-09-10", 
  "keywords": [
    "usp5", 
    "displacement assay", 
    "zinc finger", 
    "protein purification"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/a9dfcb9b-3cda-44a4-abf0-5cd791c4cfd6/1_Purification and displacement assay experiment.pdf", 
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  "identifier": "https://doi.org/10.5281/zenodo.3403804", 
  "@id": "https://doi.org/10.5281/zenodo.3403804", 
  "@type": "Dataset", 
  "name": "Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay"
}
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