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Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

Mann, Mandeep; Harding, Rachel; Schapira, Matthieu


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    <dcat:keyword>usp5</dcat:keyword>
    <dcat:keyword>displacement assay</dcat:keyword>
    <dcat:keyword>zinc finger</dcat:keyword>
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    <dct:description>&lt;p&gt;Expression and purification of&amp;nbsp;biotinylated full length (FL) USP5&lt;sup&gt;1-835&lt;/sup&gt; and biotinylated zinc finger ubiquitin-binding domain (ZnF-UBD) USP5&lt;sup&gt;171-290 &lt;/sup&gt;constructs for the development of a displacement assay.&amp;nbsp;&lt;/p&gt;</dct:description>
    <dct:description xml:lang="">Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dct:description>
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