Dataset Open Access

Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

Mann, Mandeep; Harding, Rachel; Schapira, Matthieu


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.3403804", 
  "title": "Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay", 
  "issued": {
    "date-parts": [
      [
        2019, 
        9, 
        10
      ]
    ]
  }, 
  "abstract": "<p>Expression and purification of&nbsp;biotinylated full length (FL) USP5<sup>1-835</sup> and biotinylated zinc finger ubiquitin-binding domain (ZnF-UBD) USP5<sup>171-290 </sup>constructs for the development of a displacement assay.&nbsp;</p>", 
  "author": [
    {
      "family": "Mann, Mandeep"
    }, 
    {
      "family": "Harding, Rachel"
    }, 
    {
      "family": "Schapira, Matthieu"
    }
  ], 
  "note": "Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, S\u00e3o Paulo Research Foundation-FAPESP, Takeda, and Wellcome.", 
  "type": "dataset", 
  "id": "3403804"
}
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