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Large-scale purification of Q23 and Q54 HTT-HAP40 from Sf9 expression system 2019/07/29

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


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  <identifier identifierType="DOI">10.5281/zenodo.3383265</identifier>
  <creators>
    <creator>
      <creatorName>Harding, Rachel</creatorName>
      <givenName>Rachel</givenName>
      <familyName>Harding</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-1134-391X</nameIdentifier>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Hutchinson, Ashley</creatorName>
      <givenName>Ashley</givenName>
      <familyName>Hutchinson</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Seitova, Alma</creatorName>
      <givenName>Alma</givenName>
      <familyName>Seitova</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Arrowsmith, Cheryl</creatorName>
      <givenName>Cheryl</givenName>
      <familyName>Arrowsmith</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Edwards, Aled</creatorName>
      <givenName>Aled</givenName>
      <familyName>Edwards</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Large-scale purification of Q23 and Q54 HTT-HAP40 from Sf9 expression system  2019/07/29</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2019</publicationYear>
  <subjects>
    <subject>HTT</subject>
    <subject>HAP40</subject>
    <subject>Huntingtin</subject>
    <subject>Huntington's disease</subject>
    <subject>Sf9</subject>
    <subject>Protein purification</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2019-07-29</date>
  </dates>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3383265</alternateIdentifier>
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  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.3383263</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/labscribbles</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;Project:&amp;nbsp;&lt;/strong&gt;Biophysical investigation of purified HTT protein samples&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Experiment:&amp;nbsp;&lt;/strong&gt;Large-scale purification of Q23 and Q54 HTT-HAP40 from Sf9 expression system&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Date completed:&amp;shy;&amp;nbsp;&lt;/strong&gt;2019/07/29&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Rationale:&amp;nbsp;&lt;/strong&gt;HTT samples are to be purified from different systems for subsequent biophysical and structural studies.&amp;nbsp;&lt;/p&gt;</description>
    <description descriptionType="Other">Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</description>
  </descriptions>
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