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Production of Recombinant α Synuclein Monomers and Preformed Fibrils (PFFs)

Maneca, Destiny-Love; Luo, Wen; Krahn, Andrea; Del Cid Pellitero, Esther; Shlaifer, Irina; Beitel, Lenore K.; Rao, Trisha; Durcan, Thomas M.

This protocol describes how to:
• Produce and purify recombinant human and mouse α-synuclein in monomeric form from transformed bacteria
• Generate α-synuclein preformed fibrils (PFFs) from recombinant monomeric α-synuclein
• Fluorescently label α-synuclein PFFs

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