June 15, 2019 Journal article Open Access

Mitina, Irina; Zgardan, Dan; Sturza, Rodica; Scutaru, Iurie

MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="041" ind1=" " ind2=" ">
    <subfield code="a">eng</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">red wine</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">spoilage yeast</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">DNA extraction</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">detection kit Brettanomyces/Dekkera</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">real-time PCR</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">volatile phenols</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">cycle quantification (Cq)</subfield>
  </datafield>
  <controlfield tag="005">20200120165129.0</controlfield>
  <controlfield tag="001">3249213</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">echnical University of Moldova, Department of Oenology and Chemistry, Chisinau, Republic of Moldova</subfield>
    <subfield code="a">Zgardan, Dan</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">echnical University of Moldova, Department of Oenology and Chemistry, Chisinau, Republic of Moldova</subfield>
    <subfield code="a">Sturza, Rodica</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">echnical University of Moldova, Department of Oenology and Chemistry, Chisinau, Republic of Moldova</subfield>
    <subfield code="a">Scutaru, Iurie</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">892383</subfield>
    <subfield code="z">md5:5f9a7deb3b9fba387d212ad4db68dfe0</subfield>
    <subfield code="u">https://zenodo.org/record/3249213/files/Mitina-I-JES-2019-2_pp-117-125.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2019-06-15</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="p">user-jes_utm</subfield>
    <subfield code="o">oai:zenodo.org:3249213</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="c">117-125</subfield>
    <subfield code="v">XXVI (2)</subfield>
    <subfield code="p">Journal of Engineering Science</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">The Institute of Genetics, Physiology and Plant Protection, Chisinau, Republic of Moldova</subfield>
    <subfield code="a">Mitina, Irina</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">THE METHODOLOGICAL ASPECTS OF USING REAL-TIME POLYMERASE CHAIN REACTION (RT-PCR) IN BRETTANOMYCES/DEKKERA DETECTION</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-jes_utm</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;Wine spoilage could be caused by a lot of genera and species of yeasts. One of the most damaging is &lt;em&gt;Brettanomyces/Dekkera&lt;/em&gt; which causes severe quality problems in the wine industry. Timely detection of these organisms in wine is of crucial importance for preserving wine quality. The conventional methods of diagnostics are often time-consuming and laborious. For this, molecular-based methods of rapid testing, particularly real-time PCR are gaining increasing importance. In this work, the potential of the real-time PCR method in microbiological monitoring of wines was evaluated, and analysis process according to the PIKA Weihenstephan&lt;sup&gt;TM&lt;/sup&gt; SO Detection Kit H &lt;em&gt;Brettanomyces/Dekkera&lt;/em&gt; protocol was optimized.&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.5281/zenodo.3249212</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5281/zenodo.3249213</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">article</subfield>
  </datafield>
</record>