10.5281/zenodo.3246786
https://zenodo.org/records/3246786
oai:zenodo.org:3246786
Onuh, O. A.
O. A.
Onuh
National Biotechnology Development Agency (NABDA), Along Umaru Musa Yar'adua Express Way, Airport Road, Lugbe, Abuja.
Machunga-Mambula, S. S.
S. S.
Machunga-Mambula
Department of Microbiology, Faculty of Science, University of Abuja, Gwagwalada, Nigeria.
Akin-Osanaiye, B. C.
B. C.
Akin-Osanaiye
Department of Microbiology, Faculty of Science, University of Abuja, Gwagwalada, Nigeria.
Phytochemical Screening, Chromatographic Evaluation and Antibacterial Activity of the Leaf Extracts of Lawsonia Inermis L
Zenodo
2019
Keywords: Column chromatography, Lawsonia inermis, minimum inhibitory concentration, phytochemical screening, thin-layer chromatography
2019-06-15
ang
10.5281/zenodo.3246785
Creative Commons Attribution 4.0 International
The crude methanolic extract of Lawsonia inermis leaves was subjected to chromatographic separation by column and thin layer chromatography techniques. Hexane, ethyl acetate and methanol were used as solvent system. The eluted fractions of the preliminary column chromatography gave eleven (11) fractions. All the fractions had distinct colours ranging from green to reddish brown. The eluted fractions were spotted on TLC and six brands were yielded (LT-1, LT-2, LT-3, LT-4, LT-5, LT-6). The eluted fractions were screened for antibacterial activity. The eluted fractions, LT-2 – LT-6 exhibited some degree of antibacterial activity. Furthermore, the eluted compound fractions were assayed for minimum inhibitory concentration (MIC) using the 96-well microdilution technique. Phytochemical screening of the extract revealed the presence of alkaloids, flavonoids, saponins, phenols, tannins, steroids and anthraquinone.