Journal article Open Access

Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid

Nuzzo, Genoveffa; Landi, Simone; Esercizio, Nunzia; Manzo, Emiliano; Fontana, Angelo; d'Ippolito, Giuliana


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    <subfield code="a">&lt;p&gt;The industrial production of lactic acid (LA) is mainly based on bacterial fermentation.&amp;nbsp;This process can result in enantiopure or racemic mixture according to the producing organism.&amp;nbsp;Between the enantiomers, L-lactic acid shows superior market value. Recently, we reported a novel&amp;nbsp;anaplerotic pathway called capnophilic lactic fermentation (CLF) that produces a high concentration&amp;nbsp;of LA by fermentation of sugar in the anaerobic thermophilic bacterium Thermotoga neapolitana.&amp;nbsp;The aim of this work was the&amp;nbsp;identification of the enantiomeric characterization of the LA produced&amp;nbsp;by T. neapolitana and identification of the lactate dehydrogenase in T. neapolitana (TnLDH) and related&amp;nbsp;bacteria of the order Thermotogales. Chemical derivatization and GC/MS analysis were applied to&amp;nbsp;define the stereochemistry of LA from T. neapolitana. A bioinformatics study on TnLDH was carried&lt;br&gt;
out for the characterization of the enzyme. Chemical analysis showed a 95.2% enantiomeric excess&amp;nbsp;of L-LA produced by T. neapolitana. A phylogenetic approach clearly clustered the TnLDH together&amp;nbsp;with the L-LDH from lactic acid bacteria. We report for the first time that T. neapolitana is able to&amp;nbsp;produce almost enantiopure L-lactic acid. The result was confirmed by bioinformatics analysis on&amp;nbsp;TnLDH, which is a member of the L-LDH sub-family.&lt;/p&gt;</subfield>
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