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Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid

Nuzzo, Genoveffa; Landi, Simone; Esercizio, Nunzia; Manzo, Emiliano; Fontana, Angelo; d'Ippolito, Giuliana


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  <identifier identifierType="URL">https://zenodo.org/record/3245889</identifier>
  <creators>
    <creator>
      <creatorName>Nuzzo, Genoveffa</creatorName>
      <givenName>Genoveffa</givenName>
      <familyName>Nuzzo</familyName>
    </creator>
    <creator>
      <creatorName>Landi, Simone</creatorName>
      <givenName>Simone</givenName>
      <familyName>Landi</familyName>
    </creator>
    <creator>
      <creatorName>Esercizio, Nunzia</creatorName>
      <givenName>Nunzia</givenName>
      <familyName>Esercizio</familyName>
    </creator>
    <creator>
      <creatorName>Manzo, Emiliano</creatorName>
      <givenName>Emiliano</givenName>
      <familyName>Manzo</familyName>
    </creator>
    <creator>
      <creatorName>Fontana, Angelo</creatorName>
      <givenName>Angelo</givenName>
      <familyName>Fontana</familyName>
    </creator>
    <creator>
      <creatorName>d'Ippolito, Giuliana</creatorName>
      <givenName>Giuliana</givenName>
      <familyName>d'Ippolito</familyName>
    </creator>
  </creators>
  <titles>
    <title>Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2019</publicationYear>
  <subjects>
    <subject>optical purity</subject>
    <subject>lactate dehydrogenase</subject>
    <subject>stereochemistry</subject>
    <subject>fermentation</subject>
    <subject>thermophilic bacteria</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2019-04-11</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/3245889</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.3390/fermentation5020034</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/bioreco2ver-h2020</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;The industrial production of lactic acid (LA) is mainly based on bacterial fermentation.&amp;nbsp;This process can result in enantiopure or racemic mixture according to the producing organism.&amp;nbsp;Between the enantiomers, L-lactic acid shows superior market value. Recently, we reported a novel&amp;nbsp;anaplerotic pathway called capnophilic lactic fermentation (CLF) that produces a high concentration&amp;nbsp;of LA by fermentation of sugar in the anaerobic thermophilic bacterium Thermotoga neapolitana.&amp;nbsp;The aim of this work was the&amp;nbsp;identification of the enantiomeric characterization of the LA produced&amp;nbsp;by T. neapolitana and identification of the lactate dehydrogenase in T. neapolitana (TnLDH) and related&amp;nbsp;bacteria of the order Thermotogales. Chemical derivatization and GC/MS analysis were applied to&amp;nbsp;define the stereochemistry of LA from T. neapolitana. A bioinformatics study on TnLDH was carried&lt;br&gt;
out for the characterization of the enzyme. Chemical analysis showed a 95.2% enantiomeric excess&amp;nbsp;of L-LA produced by T. neapolitana. A phylogenetic approach clearly clustered the TnLDH together&amp;nbsp;with the L-LDH from lactic acid bacteria. We report for the first time that T. neapolitana is able to&amp;nbsp;produce almost enantiopure L-lactic acid. The result was confirmed by bioinformatics analysis on&amp;nbsp;TnLDH, which is a member of the L-LDH sub-family.&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/760431/">760431</awardNumber>
      <awardTitle>Biological routes for CO2 conversion into chemical building blocks</awardTitle>
    </fundingReference>
  </fundingReferences>
</resource>
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