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Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid

Nuzzo, Genoveffa; Landi, Simone; Esercizio, Nunzia; Manzo, Emiliano; Fontana, Angelo; d'Ippolito, Giuliana


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{
  "DOI": "10.3390/fermentation5020034", 
  "container_title": "MDPI fermentation", 
  "language": "eng", 
  "title": "Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid", 
  "issued": {
    "date-parts": [
      [
        2019, 
        4, 
        11
      ]
    ]
  }, 
  "abstract": "<p>The industrial production of lactic acid (LA) is mainly based on bacterial fermentation.&nbsp;This process can result in enantiopure or racemic mixture according to the producing organism.&nbsp;Between the enantiomers, L-lactic acid shows superior market value. Recently, we reported a novel&nbsp;anaplerotic pathway called capnophilic lactic fermentation (CLF) that produces a high concentration&nbsp;of LA by fermentation of sugar in the anaerobic thermophilic bacterium Thermotoga neapolitana.&nbsp;The aim of this work was the&nbsp;identification of the enantiomeric characterization of the LA produced&nbsp;by T. neapolitana and identification of the lactate dehydrogenase in T. neapolitana (TnLDH) and related&nbsp;bacteria of the order Thermotogales. Chemical derivatization and GC/MS analysis were applied to&nbsp;define the stereochemistry of LA from T. neapolitana. A bioinformatics study on TnLDH was carried<br>\nout for the characterization of the enzyme. Chemical analysis showed a 95.2% enantiomeric excess&nbsp;of L-LA produced by T. neapolitana. A phylogenetic approach clearly clustered the TnLDH together&nbsp;with the L-LDH from lactic acid bacteria. We report for the first time that T. neapolitana is able to&nbsp;produce almost enantiopure L-lactic acid. The result was confirmed by bioinformatics analysis on&nbsp;TnLDH, which is a member of the L-LDH sub-family.</p>", 
  "author": [
    {
      "family": "Nuzzo, Genoveffa"
    }, 
    {
      "family": "Landi, Simone"
    }, 
    {
      "family": "Esercizio, Nunzia"
    }, 
    {
      "family": "Manzo, Emiliano"
    }, 
    {
      "family": "Fontana, Angelo"
    }, 
    {
      "family": "d'Ippolito, Giuliana"
    }
  ], 
  "volume": "5 (", 
  "type": "article-journal", 
  "id": "3245889"
}
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