Dataset Open Access

Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 2019/05/22

Harding, Rachel; Alvarez, Claudia; McAuley, Jacob; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


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  <dc:creator>Harding, Rachel</dc:creator>
  <dc:creator>Alvarez, Claudia</dc:creator>
  <dc:creator>McAuley, Jacob</dc:creator>
  <dc:creator>Hutchinson, Ashley</dc:creator>
  <dc:creator>Seitova, Alma</dc:creator>
  <dc:creator>Arrowsmith, Cheryl</dc:creator>
  <dc:creator>Edwards, Aled</dc:creator>
  <dc:date>2019-05-22</dc:date>
  <dc:description>Project: Biophysical investigation of purified HTT protein samples

Experiment: Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography

Date completed:­ 2019/05/22

Rationale: Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In small-scale experiment, I also incorporated helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment included 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step and yielded a highly pure HTT-HAP40 sample: https://zenodo.org/record/3234174.I now want to try this purification with apo HTT. </dc:description>
  <dc:identifier>https://zenodo.org/record/3234182</dc:identifier>
  <dc:identifier>10.5281/zenodo.3234182</dc:identifier>
  <dc:identifier>oai:zenodo.org:3234182</dc:identifier>
  <dc:relation>doi:10.5281/zenodo.3234181</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/labscribbles</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/openlabnotebooks</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>Huntingtin</dc:subject>
  <dc:subject>HTT</dc:subject>
  <dc:subject>HAP40</dc:subject>
  <dc:subject>Huntington's disease</dc:subject>
  <dc:subject>HD</dc:subject>
  <dc:title>Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 2019/05/22</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
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