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Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 2019/05/22

Harding, Rachel; Alvarez, Claudia; McAuley, Jacob; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


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{
  "description": "<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein samples</p>\n\n<p><strong>Experiment:&nbsp;</strong>Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography</p>\n\n<p><strong>Date completed:&shy;&nbsp;</strong>2019/05/22</p>\n\n<p><strong>Rationale:&nbsp;</strong>Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In small-scale experiment, I also incorporated helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment included 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step and yielded a highly pure HTT-HAP40 sample:&nbsp;https://zenodo.org/record/3234174.I now want to try this purification with apo HTT.&nbsp;</p>", 
  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Alvarez, Claudia"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada", 
      "@type": "Person", 
      "name": "McAuley, Jacob"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada", 
      "@type": "Person", 
      "name": "Hutchinson, Ashley"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada", 
      "@type": "Person", 
      "name": "Seitova, Alma"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto, MaRS South Tower, 101 College Street, Toronto, Ontario M5G 1L7, Canada", 
      "@type": "Person", 
      "name": "Edwards, Aled"
    }
  ], 
  "url": "https://zenodo.org/record/3234182", 
  "datePublished": "2019-05-22", 
  "keywords": [
    "Huntingtin", 
    "HTT", 
    "HAP40", 
    "Huntington's disease", 
    "HD"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/b37bb907-3eaf-4093-a501-ca7efbbd8eea/Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 20190522.docx", 
      "@type": "DataDownload", 
      "fileFormat": "docx"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/b37bb907-3eaf-4093-a501-ca7efbbd8eea/Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 20190522.pdf", 
      "@type": "DataDownload", 
      "fileFormat": "pdf"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.3234182", 
  "@id": "https://doi.org/10.5281/zenodo.3234182", 
  "@type": "Dataset", 
  "name": "Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 2019/05/22"
}
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