Dataset Open Access

Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

Harding, Rachel; Deme, Justin; Lea, Susan; Arrowsmith, Cheryl; Edwards, Aled


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nmm##2200000uu#4500</leader>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Huntingtin</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">HTT</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">HD</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Huntington's disease</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">CryoEM</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Phosphorylation</subfield>
  </datafield>
  <controlfield tag="005">20191101071112.0</controlfield>
  <datafield tag="500" ind1=" " ind2=" ">
    <subfield code="a">Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</subfield>
  </datafield>
  <controlfield tag="001">3234167</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Oxford University</subfield>
    <subfield code="a">Deme, Justin</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Oxford University</subfield>
    <subfield code="a">Lea, Susan</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Structural Genomics Consortium, University of Toronto</subfield>
    <subfield code="a">Arrowsmith, Cheryl</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Structural Genomics Consortium, University of Toronto</subfield>
    <subfield code="a">Edwards, Aled</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">1301193</subfield>
    <subfield code="z">md5:01b87cf3d49e40b0fe75b0b01fcfa4fe</subfield>
    <subfield code="u">https://zenodo.org/record/3234167/files/Analysis of dephosphorylated HTT samples by cryoEM analysis  20190501.docx</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">441969</subfield>
    <subfield code="z">md5:d4fdc40c9e69339bc7ea44e7c1d1631d</subfield>
    <subfield code="u">https://zenodo.org/record/3234167/files/Analysis of dephosphorylated HTT samples by cryoEM analysis  20190501.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2019-05-01</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">user-labscribbles</subfield>
    <subfield code="p">user-openlabnotebooks</subfield>
    <subfield code="p">user-sgc-opennotebook</subfield>
    <subfield code="o">oai:zenodo.org:3234167</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">Structural Genomics Consortium, University of Toronto</subfield>
    <subfield code="0">(orcid)0000-0002-1134-391X</subfield>
    <subfield code="a">Harding, Rachel</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-labscribbles</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-openlabnotebooks</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-sgc-opennotebook</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">http://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;&lt;strong&gt;Project:&amp;nbsp;&lt;/strong&gt;High resolution structural analysis of purified HTT samples&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Experiment:&amp;nbsp;&lt;/strong&gt;Analysis of dephosphorylated HTT samples by cryoEM analysis&amp;nbsp;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Date completed:&amp;shy;&amp;nbsp;&lt;/strong&gt;2019/05/01&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Rationale:&amp;nbsp;&lt;/strong&gt;We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM.&amp;nbsp;&lt;/p&gt;

&lt;p&gt;&amp;nbsp;&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.5281/zenodo.3234166</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5281/zenodo.3234167</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">dataset</subfield>
  </datafield>
</record>
51
38
views
downloads
All versions This version
Views 5151
Downloads 3838
Data volume 24.5 MB24.5 MB
Unique views 3737
Unique downloads 2525

Share

Cite as