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Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

Harding, Rachel; Deme, Justin; Lea, Susan; Arrowsmith, Cheryl; Edwards, Aled


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  <dc:creator>Harding, Rachel</dc:creator>
  <dc:creator>Deme, Justin</dc:creator>
  <dc:creator>Lea, Susan</dc:creator>
  <dc:creator>Arrowsmith, Cheryl</dc:creator>
  <dc:creator>Edwards, Aled</dc:creator>
  <dc:date>2019-05-01</dc:date>
  <dc:description>Project: High resolution structural analysis of purified HTT samples

Experiment: Analysis of dephosphorylated HTT samples by cryoEM analysis 

Date completed:­ 2019/05/01

Rationale: We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM. 

 </dc:description>
  <dc:identifier>https://zenodo.org/record/3234167</dc:identifier>
  <dc:identifier>10.5281/zenodo.3234167</dc:identifier>
  <dc:identifier>oai:zenodo.org:3234167</dc:identifier>
  <dc:relation>doi:10.5281/zenodo.3234166</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/labscribbles</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/openlabnotebooks</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>Huntingtin</dc:subject>
  <dc:subject>HTT</dc:subject>
  <dc:subject>HD</dc:subject>
  <dc:subject>Huntington's disease</dc:subject>
  <dc:subject>CryoEM</dc:subject>
  <dc:subject>Phosphorylation</dc:subject>
  <dc:title>Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
</oai_dc:dc>
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