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Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

Harding, Rachel; Deme, Justin; Lea, Susan; Arrowsmith, Cheryl; Edwards, Aled


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{
  "description": "<p><strong>Project:&nbsp;</strong>High resolution structural analysis of purified HTT samples</p>\n\n<p><strong>Experiment:&nbsp;</strong>Analysis of dephosphorylated HTT samples by cryoEM analysis&nbsp;</p>\n\n<p><strong>Date completed:&shy;&nbsp;</strong>2019/05/01</p>\n\n<p><strong>Rationale:&nbsp;</strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM.&nbsp;</p>\n\n<p>&nbsp;</p>", 
  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Oxford University", 
      "@type": "Person", 
      "name": "Deme, Justin"
    }, 
    {
      "affiliation": "Oxford University", 
      "@type": "Person", 
      "name": "Lea, Susan"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Edwards, Aled"
    }
  ], 
  "url": "https://zenodo.org/record/3234167", 
  "datePublished": "2019-05-01", 
  "keywords": [
    "Huntingtin", 
    "HTT", 
    "HD", 
    "Huntington's disease", 
    "CryoEM", 
    "Phosphorylation"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/7aea8a60-eaa8-4fba-895a-ec3233ae1e5d/Analysis of dephosphorylated HTT samples by cryoEM analysis  20190501.docx", 
      "@type": "DataDownload", 
      "fileFormat": "docx"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/7aea8a60-eaa8-4fba-895a-ec3233ae1e5d/Analysis of dephosphorylated HTT samples by cryoEM analysis  20190501.pdf", 
      "@type": "DataDownload", 
      "fileFormat": "pdf"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.3234167", 
  "@id": "https://doi.org/10.5281/zenodo.3234167", 
  "@type": "Dataset", 
  "name": "Analysis of dephosphorylated HTT samples by cryoEM analysis\u00a02019/05/01"
}
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