Journal article Open Access

Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum

Othman, Nasim A. R. M.


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    <subfield code="a">Antioxidant enzymes</subfield>
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    <subfield code="a">Carbohydrates</subfield>
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    <subfield code="a">Copper</subfield>
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    <subfield code="a">Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum</subfield>
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    <subfield code="a">&lt;p&gt;Here was investigated the incorporation of copper in MS medium on growth, and metabolic activities of &lt;em&gt;Solanum nigrum&lt;/em&gt; callus. Copper up to 75 &amp;micro;M increased the growth, and thereafter a decline was observed. No considerable alteration in MDA, H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;, bound phenolics, flavonoids, ascorbate, and copper content was observed with the existence of 25 &amp;micro;M copper, then levels of these parameters were raised with rising copper concentrations. Similarly, 25 &amp;micro;M copper didn&amp;#39;t induce a considerable change in lipoxygenase, superoxide dismutase, catalase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase activities, however, high levels stimulated these enzymes. Copper at 25 &amp;micro;M didn&amp;rsquo;t considerably reduce amino acids and soluble proteins, whereas higher concentrations reduced these parameters. Copper treatments reduced the soluble carbohydrates accumulation; only 75 &amp;micro;M enhanced this accumulation. Copper at 25 &amp;micro;M significantly increased the potassium accumulation, whereas higher concentrations reduced this accumulation. From these results, it might be contemplated the optimum effect concerning copper.&lt;/p&gt;</subfield>
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