Journal article Open Access

Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum

Othman, Nasim A. R. M.

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  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.2848646", 
  "container_title": "European Journal of Biological Research", 
  "title": "Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum", 
  "issued": {
    "date-parts": [
  "abstract": "<p>Here was investigated the incorporation of copper in MS medium on growth, and metabolic activities of <em>Solanum nigrum</em> callus. Copper up to 75 &micro;M increased the growth, and thereafter a decline was observed. No considerable alteration in MDA, H<sub>2</sub>O<sub>2</sub>, bound phenolics, flavonoids, ascorbate, and copper content was observed with the existence of 25 &micro;M copper, then levels of these parameters were raised with rising copper concentrations. Similarly, 25 &micro;M copper didn&#39;t induce a considerable change in lipoxygenase, superoxide dismutase, catalase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase activities, however, high levels stimulated these enzymes. Copper at 25 &micro;M didn&rsquo;t considerably reduce amino acids and soluble proteins, whereas higher concentrations reduced these parameters. Copper treatments reduced the soluble carbohydrates accumulation; only 75 &micro;M enhanced this accumulation. Copper at 25 &micro;M significantly increased the potassium accumulation, whereas higher concentrations reduced this accumulation. From these results, it might be contemplated the optimum effect concerning copper.</p>", 
  "author": [
      "family": "Othman, Nasim A. R. M."
  "page": "77-92", 
  "volume": "9", 
  "type": "article-journal", 
  "issue": "2", 
  "id": "2848646"
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