Dataset Open Access
Chee Huat (Linus) Eng; Long Cai
{ "description": "<p>These source data consist of the point locations of individual decoded mRNA dots in seqFISH+ experiments on NIH/3T3 cells. DAPI_experiment folder contains the DAPI stainings with other beads images. ROIs_experiment folders are the manual segmentation performed in ImageJ. We only performed segmentation on the whole single cells. In seqFISH+_NIH3T3_point_locations zip folder, there are 3 matlab files . One is gene name file, the other two files are point locations for replicate 1 and 2 seqFISH+ experiments in NIH/3T3 cells. The columns of the data represent field of view(FOV), Cell , and Gene. For example : FOV1 , Cell 5, Gene : Bgn to retrieve the point locations. </p>", "license": "https://creativecommons.org/licenses/by/4.0/legalcode", "creator": [ { "affiliation": "California Institute of Technology", "@type": "Person", "name": "Chee Huat (Linus) Eng" }, { "affiliation": "California Institute of Technology", "@type": "Person", "name": "Long Cai" } ], "url": "https://zenodo.org/record/2669683", "datePublished": "2019-05-06", "keywords": [], "@context": "https://schema.org/", "distribution": [ { "contentUrl": "https://zenodo.org/api/files/3083c6f7-6bf9-47c9-8128-bf6550a6d81f/DAPI_experiment1.zip", "encodingFormat": "zip", "@type": "DataDownload" }, { "contentUrl": "https://zenodo.org/api/files/3083c6f7-6bf9-47c9-8128-bf6550a6d81f/DAPI_experiment2.zip", "encodingFormat": "zip", "@type": "DataDownload" }, { "contentUrl": "https://zenodo.org/api/files/3083c6f7-6bf9-47c9-8128-bf6550a6d81f/ROIs_Experiment1_NIH3T3.zip", "encodingFormat": "zip", "@type": "DataDownload" }, { "contentUrl": "https://zenodo.org/api/files/3083c6f7-6bf9-47c9-8128-bf6550a6d81f/ROIs_Experiment2_NIH3T3.zip", "encodingFormat": "zip", "@type": "DataDownload" }, { "contentUrl": "https://zenodo.org/api/files/3083c6f7-6bf9-47c9-8128-bf6550a6d81f/seqFISH+_NIH3T3_point_locations.zip", "encodingFormat": "zip", "@type": "DataDownload" } ], "identifier": "https://doi.org/10.5281/zenodo.2669683", "@id": "https://doi.org/10.5281/zenodo.2669683", "@type": "Dataset", "name": "NIH3T3_point_locations for RNA seqFISH+ experiments" }
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