May 6, 2019 Dataset Open Access

Chee Huat (Linus) Eng; Long Cai

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  <identifier identifierType="DOI">10.5281/zenodo.2669683</identifier>
  <creators>
    <creator>
      <creatorName>Chee Huat (Linus) Eng</creatorName>
      <affiliation>California Institute of Technology</affiliation>
    </creator>
    <creator>
      <creatorName>Long Cai</creatorName>
      <affiliation>California Institute of Technology</affiliation>
    </creator>
  </creators>
  <titles>
    <title>NIH3T3_point_locations for RNA seqFISH+ experiments</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2019</publicationYear>
  <dates>
    <date dateType="Issued">2019-05-06</date>
  </dates>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/2669683</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.2669682</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;These source data consist of the point locations of individual decoded mRNA dots in seqFISH+ experiments on NIH/3T3 cells. DAPI_experiment folder contains the DAPI stainings with other beads images. ROIs_experiment folders are the manual segmentation performed in ImageJ. We only performed segmentation on the whole single cells. In seqFISH+_NIH3T3_point_locations zip folder, there are 3 matlab files .&amp;nbsp;One is gene name file, the other two files are point locations for replicate 1 and 2 seqFISH+ experiments in&amp;nbsp;NIH/3T3 cells. The columns of the data represent field of view(FOV), Cell , and Gene. For example :&amp;nbsp;FOV1 , Cell 5, Gene : Bgn to retrieve the point locations.&amp;nbsp;&lt;/p&gt;</description>
    <description descriptionType="Other">{"references": ["Eng, C.-H. L. et al. Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+. Nature 568, 235\u2013239 (2019)"]}</description>
  </descriptions>
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