Metagenomes of blood and psoriatic skin. Research project.

The first stage of the project devoted to the study, diagnostics and treatment of psoriasis is substantiated and given a detailed description. The purpose of the first stage is testing the basic hypotheses of new systemic YN-model of psoriasis pathogenesis. The test consists in complex studying of whole blood metagenome and metagenome of psoriatic skin (phagocytes). Metagenome is all non-host DNA (here - non-human) which is found in biomaterial. The necessary algorithms for complex studying of metagenomes are suggested and substantiated.

Survey and analytical assessment of the results of fundamental works on studying metagenomes of blood and skin is carried out. All works (published prior to the beginning of 2019) on determining bacterial DNA concentration in whole blood of healthy people are reviewed.

Detailed comparison of characteristics of 16S-test and WMS-test is carried out.

The objectives and tasks (including the order of solving them) of the first stage are formulated. The main questions are raised, to which the results of the first stage are supposed to give answers. The order of preparing and implementing WMS-test of whole blood is suggested.

Detailed comparison of YN-model of pathogenesis with the previously published  Y-model is conducted. An analytical review of works on studying the attraction of blood neutrophils in psoriatic skin and their possible subsequent netosis is made. Netosis in psoriatic skin of neutrophils which are carriers of endocytosed Y-antigen in blood is qualified as an essential link in the vicious cycle of psoriatic inflammation within YN-model. Classification of psoriatic disease as netopathy is suggested.

Psoriagenic bacteria are given definition based on the existence of genes responsible for the formation of interpeptide bridges. The smallest peptides (peptidoglycan fragments) - potential Y-antigens are identified.

The illustrations are in the form of a presentation and are in a separate pdf-file (Supplement A):
https://doi.org/10.5281/zenodo.2668376

Supplements S1-S8 are in a separate pdf-file also (Supplement B):
https://doi.org/10.5281/zenodo.2668459

The given book is the authorized translation of the book (edition r2.2), published in Russian:   https://doi.org/10.5281/zenodo.2677815 (with Supplements A, B and C).