2640991
doi
10.5281/zenodo.2640991
oai:zenodo.org:2640991
van der Sman, Piret
Agricultural Research Centre (ARC), Harjumaa, Estonia
Järvekülg, Lilian
Tallin University of Technology (TTU), Tallin, Estonia
Grausgruber-Gröger, Sabine
Austrian Agency for Health and Food Safety (AGES), Vienna, Austria
Renvoisé, Jean-Philippe
FRENCH AGENCY FOR FOOD, ENVIRONMENTAL AND OCCUPATIONAL HEALTH & SAFETY (ANSES), Lempdes, France
Tomassoli, Laura
COUNCIL FOR THE RESEARCH IN AGRICULTURE AND THE ANALYSIS OF THE AGRICULTURAL ECONOMY (CREA), Rome, Italy
Glais, Laurent
THE NATIONAL POTATO PLANT GROWERS ASSOCIATION IN FRANCE (FN3PT), Le Rheu, France
Smith, Linda
NEDERLANDSE ALGEMEME KEURINGSDIENST (NAK), Emmeloord, the Netherlands
Ring test for improved Potato virus Y strain detection
Lasner, Helena
Agricultural Research Centre (ARC), Harjumaa, Estonia
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Euphresco, plant health, potato, Potato virus Y, diagnostics, ELISA, RT-PCR, RT real-time PCR, interlaboratory comparison
<p><em>Potato virus Y</em> (PVY) is one of the most damaging virus in potato industries and it can cause crop loss up to 80%. Several strains of PVY have been identified that differ by the symptoms they cause. PVY<sup>O</sup>, the common strain causes mosaic symptoms; PVY<sup>C</sup> that causes stipple streak; PVY<sup>N</sup>, the necrotic strain that causes mild foliage symptoms, but necrosis in tobacco plants. Mixed infections of common and necrotic strains are frequent, and genomes can mix, producing recombinant strains. Over the last years, several reports (Davie <em>et al.</em> 2017, Gray <em>et al</em>. 2010,<sup> </sup>Karasev <em>et al.</em> 2013,<sup> </sup>MacKenzie <em>et al.</em> 2018,<sup> </sup>Elwan <em>et al.</em> 2017, Chikh Ali <em>et al</em>. 2013) from all over the world are declaring that recombinant strains are becoming dominant. Those recombinant strains, mostly PVY<sup>N-Wi </sup>(PVY<sup>N:O</sup>) and PVY<sup>NTN</sup> (main cause of tuber necrosis) are of increasing importance in European seed stocks. Symptoms on plants alone cannot distinguish these virus strains. Diagnosis between the different PVY strains can be difficult, as the widely used serological method (ELISA) is only able to distinguish PVY<sup> </sup>strain between O- and N-serotype but is unable to distinguish recombinant strains from non-recombinant. Recent emergence of genetically recombinant and serologically different strains of PVY has led to the development of several diagnostic protocols to determine strain identity and to detect mixed strain infections including simplex and multiplex PCR assays.<em> </em>Other approaches include a combination of immunological and molecular methods<em>, </em>though no common method for the detection of PVY and its strains has been adopted by the pathogen detection laboratories within Europe.</p>
<p>The main aim of the project was to develop and validate a protocol for the detection and identification of <em>Potato virus Y</em> and its associated strains. During the project different PVY strain identification tests described in published scientific articles were considered and data on these tests were provided by project partners in order to select those that allowed PVY wide range strain identification; an interlaboratory comparison was organized and a validated protocol was agreed and is presented in Annex 1.</p>
Report of the Euphresco project 2015-B-148 Ring test for improved Potato virus Y strain detection
Zenodo
2019-04-15
info:eu-repo/semantics/report
2640990
1579537401.109428
568784
md5:449670829781e3dc24e4a02135486abe
https://zenodo.org/records/2640991/files/Report_2015-B-148_FINAL.pdf
public
10.5281/zenodo.2640990
isVersionOf
doi