{ "access": { "embargo": { "active": false, "reason": null }, "files": "public", "record": "public", "status": "open" }, "created": "2019-04-15T14:27:17.096855+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "files": { "count": 1, "enabled": true, "entries": { "Expansion and Diversification of MFS Transporters in Kluyveromyces marxianus.pdf": { "checksum": "md5:fd3f24a5f898deb4e6d48dba88754f36", "ext": "pdf", "id": "d7661029-d5be-44cc-84a0-824bb9385b3f", "key": "Expansion and Diversification of MFS Transporters in Kluyveromyces marxianus.pdf", "metadata": null, "mimetype": "application/pdf", "size": 8515476 } }, "order": [], "total_bytes": 8515476 }, "id": "2640876", "is_draft": false, "is_published": true, "links": { "access": "https://zenodo.org/api/records/2640876/access", "access_links": "https://zenodo.org/api/records/2640876/access/links", "access_request": "https://zenodo.org/api/records/2640876/access/request", "access_users": "https://zenodo.org/api/records/2640876/access/users", "archive": "https://zenodo.org/api/records/2640876/files-archive", "archive_media": "https://zenodo.org/api/records/2640876/media-files-archive", "communities": "https://zenodo.org/api/records/2640876/communities", "communities-suggestions": "https://zenodo.org/api/records/2640876/communities-suggestions", "doi": "https://doi.org/10.3389/fmicb.2018.03330", "draft": "https://zenodo.org/api/records/2640876/draft", "files": "https://zenodo.org/api/records/2640876/files", "latest": "https://zenodo.org/api/records/2640876/versions/latest", "latest_html": "https://zenodo.org/records/2640876/latest", "media_files": "https://zenodo.org/api/records/2640876/media-files", "parent": "https://zenodo.org/api/records/2640875", "parent_doi": "https://zenodo.org/doi/", "parent_html": "https://zenodo.org/records/2640875", "requests": "https://zenodo.org/api/records/2640876/requests", "reserve_doi": "https://zenodo.org/api/records/2640876/draft/pids/doi", "self": "https://zenodo.org/api/records/2640876", "self_doi": "https://zenodo.org/doi/10.3389/fmicb.2018.03330", "self_html": "https://zenodo.org/records/2640876", "self_iiif_manifest": "https://zenodo.org/api/iiif/record:2640876/manifest", "self_iiif_sequence": "https://zenodo.org/api/iiif/record:2640876/sequence/default", "versions": "https://zenodo.org/api/records/2640876/versions" }, "media_files": { "count": 0, "enabled": false, "entries": {}, "order": [], "total_bytes": 0 }, "metadata": { "creators": [ { "affiliations": [ { "name": "School of Microbiology, Centre for Synthetic Biology and Biotechnology, Environmental Research Institute, APC Microbiome Institute, University College Cork, Cork, Ireland" } ], "person_or_org": { "family_name": "Varela", "given_name": "Javier A", "name": "Varela, Javier A", "type": "personal" } }, { "affiliations": [ { "name": "School of Microbiology, Centre for Synthetic Biology and Biotechnology, Environmental Research Institute, APC Microbiome Institute, University College Cork, Cork, Ireland" } ], "person_or_org": { "family_name": "Puricelli", "given_name": "Martina", "name": "Puricelli, Martina", "type": "personal" } }, { "affiliations": [ { "name": "School of Microbiology, Centre for Synthetic Biology and Biotechnology, Environmental Research Institute, APC Microbiome Institute, University College Cork, Cork, Ireland" } ], "person_or_org": { "family_name": "Montini", "given_name": "Noemi", "name": "Montini, Noemi", "type": "personal" } }, { "affiliations": [ { "name": "School of Microbiology, Centre for Synthetic Biology and Biotechnology, Environmental Research Institute, APC Microbiome Institute, University College Cork, Cork, Ireland" } ], "person_or_org": { "family_name": "Morrissey", "given_name": "John P", "name": "Morrissey, John P", "type": "personal" } } ], "description": "
In yeasts, proteins of the Major Superfamily Transporter selectively bind and allow the uptake of sugars to permit growth on varied substrates. The genome of brewer’s yeast, Saccharomyces cerevisiae, encodes multiple hexose transporters (Hxt) to transport glucose and other MFS proteins for maltose, galactose, and other monomers. For sugar uptake, the dairy yeast, Kluyveromyces lactis, uses Rag1p for glucose, Hgt1 for glucose and galactose, and Lac12 for lactose. In the related industrial species Kluyveromyces marxianus, there are four genes encoding Lac12-like proteins but only one of them, Lac12, can transport lactose. In this study, which initiated with efforts to investigate possible functions encoded by the additional LAC12 genes in K. marxianus, a genomewide survey of putative MFS sugar transporters was performed. Unexpectedly, it was found that the KHT and the HGT genes are present as tandem arrays of five to six copies, with the precise number varying between isolates. Heterologous expression of individual genes in S. cerevisiae and mutagenesis of single and multiple genes in K. marxianus was performed to establish possible substrates for these transporters. The focus was on the sugar galactose since it was already reported in K. lactis that this hexose was a substrate for both Lac12 and Hgt1. It emerged that three of the four copies of Lac12, four Hgt-like proteins and one Kht-like protein have some capacity to transport galactose when expressed in S. cerevisiae and inactivation of all eight genes was required to completely abolish galactose uptake in K. marxianus. Analysis of the amino acid sequence of all known yeast galactose transporters failed to identify common residues that explain the selectivity for galactose. Instead, the capacity to transport galactose has arisen three different times in K. marxianus via polymorphisms in proteins that are probably ancestral glucose transporters. Although, this is analogous to S. cerevisiae, in which Gal2 is related to glucose transporters, there are not conserved amino acid changes, either with Gal2, or among the K. marxianus galactose transporters. The data highlight how gene duplication and functional diversification has provided K. marxianus with versatile capacity to utilise sugars for growth.
", "funding": [ { "award": { "acronym": "CHASSY", "id": "00k4n6c32::720824", "identifiers": [ { "identifier": "https://cordis.europa.eu/projects/720824", "scheme": "url" } ], "number": "720824", "program": "H2020", "title": { "en": "Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds" } }, "funder": { "id": "00k4n6c32", "name": "European Commission" } } ], "publication_date": "2019-01-10", "publisher": "Zenodo", "resource_type": { "id": "publication-article", "title": { "de": "Zeitschriftenartikel", "en": "Journal article" } }, "rights": [ { "description": { "en": "The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited." }, "icon": "cc-by-icon", "id": "cc-by-4.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/licenses/by/4.0/legalcode" }, "title": { "en": "Creative Commons Attribution 4.0 International" } } ], "title": "Expansion and Diversification of MFS Transporters in Kluyveromyces marxianus" }, "parent": { "access": { "owned_by": { "user": 25503 } }, "communities": { "default": "05079b33-4f63-4bb8-b3d0-8319e1d18400", "entries": [ { "access": { "member_policy": "open", "members_visibility": "public", "record_policy": "open", "review_policy": "open", "visibility": "public" }, "children": { "allow": false }, "created": "2017-03-08T10:51:47.067482+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "05079b33-4f63-4bb8-b3d0-8319e1d18400", "links": {}, "metadata": { "curation_policy": "", "page": "This is the collection of materials created within the "CHASSY: Model-Based Construction and Optimisation of Versatile Chassis Yeast Strains for Production of Valuable Lipid and Aromatic Compounds", H2020 Research Innovation Action (Grant Agreement 720824). http://www.chassy.eu/
\r\n\r\nThe aim of CHASSY is to deliver a suite of yeast strains that can serve as versatile platforms for the production of high value oleochemicals and aromatic molecules. This will be achieved by integrating the knowledge gained from systems biology with the engineering tools of synthetic biology to redesign metabolic pathways in the target yeast species. These redesigned strains will have optimised levels of product precursors and will serve as versatile chassis for industrial exploitation.
\r\n\r\nCHASSY is a 10-partner European project (grant number 720824) funded under the Industrial Leadership Priority of the EU Horizon 2020 Programme. More specifically, it was approved for funding under the Biotechnology Topic of the 2016 Leadership in Enabling and Industrial Technologies call. Biotechnology has been identified as on of the Key Enabling Technologies required to sustain European industrial competitiveness.
\r\n\r\nThe specific objectives of the CHASSY are to:
\r\n\r\nUse comparative systems biology to understand and redesign the core metabolic networks of S. cerevisiae Y. lipolytica and K. marxianus to develop highly efficient chassis strains
\r\n\r\nBased on the GEMs, build new yeast platforms that will serve as chassis for the development of versatile, robust cell factories for products derived from the fatty acid and shikimate pathways
\r\n\r\nDevelop efficient, prototype yeast cell factory strains producing high value lipids and aromatics
\r\n\r\nTransfer knowledge and concepts of cutting-edge systems biology-driven design and construction of yeast cell factories to European SMEs
\r\n\r\n", "title": "CHASSY H2020 Project" }, "revision_id": 0, "slug": "chassy-h2020", "updated": "2017-03-08T15:03:18.960677+00:00" } ], "ids": [ "05079b33-4f63-4bb8-b3d0-8319e1d18400" ] }, "id": "2640875", "pids": {} }, "pids": { "doi": { "identifier": "10.3389/fmicb.2018.03330", "provider": "external" }, "oai": { "identifier": "oai:zenodo.org:2640876", "provider": "oai" } }, "revision_id": 4, "stats": { "all_versions": { "data_volume": 860063076.0, "downloads": 101, "unique_downloads": 100, "unique_views": 133, "views": 141 }, "this_version": { "data_volume": 860063076.0, "downloads": 101, "unique_downloads": 100, "unique_views": 133, "views": 141 } }, "status": "published", "updated": "2020-01-20T16:51:59.233021+00:00", "versions": { "index": 1, "is_latest": true } }