Dataset Open Access
Project: High resolution structural analysis of purified HTT proteins
Experiment: Cloning and test expression of HTT fragment clones
Date completed: 2019/02/28
Rationale: I aim to complement our previous work on purifying stable regions of huntingtin by cloning further regions of huntingtin corresponding to discrete HEAT repeats for eukaryotic expression. Constructs will initially be screened by BVES in insect sf9 cells. High quality constructs will undergo extensive crystallization experiments and structure determination by X-ray crystallography using well established high-throughput and systematic protocols in place at the SGC. Any solved structures may allow generation of a pseudoatomic resolution (<4 Å) model of the HTT protein using the cryoEM model as a guide.
Cloning and test expression of HTT fragment clones 20190228.docx
Cloning and test expression of HTT fragment clones 20190228.pdf