10.5281/zenodo.2628967
https://zenodo.org/records/2628967
oai:zenodo.org:2628967
Hiroaki Adachi
Hiroaki Adachi
0000-0002-7184-744X
The Sainsbury Laboratory
Sophien Kamoun
Sophien Kamoun
0000-0002-0290-0315
The Sainsbury Laboratory
Abbas Maqbool
Abbas Maqbool
0000-0002-6189-5560
The Sainsbury Laboratory
Death switch: how resistosomes activate immunity
Zenodo
2019
immunity
plants
pathogens
biophysics
biochemistry
2019-04-04
eng
10.5281/zenodo.2628966
https://zenodo.org/communities/openplantnlr
https://zenodo.org/communities/kamounlab
Creative Commons Attribution 4.0 International
Now published in Nature Plants, 5:457-458
Pathogen perception triggers a monomeric NLR plant immune receptor to form a pentameric wheel-like complex termed a resistosome, with the N-terminal ahelices forming a funnel-shaped structure that may perturb plasma membrane integrity to cause hypersensitive cell death.
Nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular receptors that detect pathogen molecules and activate a robust immune response that includes the hypersensitive response (HR) cell death in plants. Although plant NLRs are known to oilgomerize through their N-terminal domains, such as the coiled coil (CC) domain, the molecular mechanisms that underpin plant NLR activation and the subsequent execution of HR cell death have remained largely unknown. In two recent paradigm-shifting Science papers, Wang et al. have advanced our understanding of both the structural and biochemical bases of NLR activation in plants, creating a new conceptual framework. They reconstituted both the inactive and active complexes of the CC-NLR protein HOPZ-ACTIVATED RESISTANCE1 (ZAR1) with its partner receptor-like cytoplasmic kinases (RLCKs) and used cryo-electron microscopy to show that activated ZAR1 forms a resistosome—a wheel-like pentamer that can switch conformation to expose a funnel-shaped structure formed by the very N-terminal ahelices (a1) of the CC domains. They propose a provocative model in which the five exposeda1 helices of the ZAR1 resistosome form a funnel-like structurethat triggers HR cell death by translocating into the plasma membrane and perturbing membrane integrity similar to pore-forming toxins.
European Commission
10.13039/501100000780
743165
Retooling plant immunity for resistance to blast fungi
European Commission
10.13039/501100000780
294608
Next generation disease resistance breeding in plants