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Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/04/02

Harding, Rachel; Arrowsmith, Cheryl; Edwards, Aled


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  <dc:creator>Harding, Rachel</dc:creator>
  <dc:creator>Arrowsmith, Cheryl</dc:creator>
  <dc:creator>Edwards, Aled</dc:creator>
  <dc:date>2019-04-02</dc:date>
  <dc:description>Project: Biophysical investigation of purified HTT protein samples

Experiment: Optimisation of HTT-HAP40 purification using heparin affinity chromatography

Date completed:­ 2019/04/02

Rationale: Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In addition, I will incorporate helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment will include 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step. </dc:description>
  <dc:identifier>https://zenodo.org/record/2628064</dc:identifier>
  <dc:identifier>10.5281/zenodo.2628064</dc:identifier>
  <dc:identifier>oai:zenodo.org:2628064</dc:identifier>
  <dc:relation>doi:10.5281/zenodo.2628063</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/labscribbles</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/openlabnotebooks</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>Huntingtin</dc:subject>
  <dc:subject>Huntington's disease</dc:subject>
  <dc:subject>Protein purification</dc:subject>
  <dc:subject>BVES</dc:subject>
  <dc:title>Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/04/02</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
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