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Preparation of dephosphorylated HTT samples for cryoEM analysis 2019/03/20

Harding, Rachel; Duan, Shili; Arrowsmith, Cheryl


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{
  "description": "<p><strong>Project:&nbsp;</strong>High resolution structural analysis of purified HTT samples</p>\n\n<p><strong>Experiment:&nbsp;</strong>Preparation of dephosphorylated HTT samples for cryoEM analysis</p>\n\n<p><strong>Date completed:&shy;&nbsp;</strong>2019/03/20</p>\n\n<p><strong>Rationale:&nbsp;</strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM.&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada", 
      "@type": "Person", 
      "name": "Duan, Shili"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }
  ], 
  "url": "https://zenodo.org/record/2601987", 
  "datePublished": "2019-03-20", 
  "keywords": [
    "Huntingtin", 
    "Huntington's disease", 
    "HTT", 
    "GRAFIX", 
    "CryoEM"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
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      "contentUrl": "https://zenodo.org/api/files/ef746805-d2f6-495d-8c6c-22d02a18c113/Preparation of dephosphorylated HTT samples for cryoEM analysis 20190320.docx", 
      "encodingFormat": "docx", 
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      "contentUrl": "https://zenodo.org/api/files/ef746805-d2f6-495d-8c6c-22d02a18c113/Preparation of dephosphorylated HTT samples for cryoEM analysis 20190320.pdf", 
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  "identifier": "https://doi.org/10.5281/zenodo.2601987", 
  "@id": "https://doi.org/10.5281/zenodo.2601987", 
  "@type": "Dataset", 
  "name": "Preparation of dephosphorylated HTT samples for cryoEM analysis 2019/03/20"
}
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