Dataset Open Access
Harding, Rachel;
Arrowsmith, Cheryl;
Edwards, Aled
Project: Biophysical investigation of purified HTT protein samples
Experiment: Optimisation of HTT-HAP40 purification using heparin affinity chromatography
Date completed: 2019/01/29
Rationale: The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems were conducted using heparin and FLAG affinity chromatography which showed the sample bound heparin resin – see https://zenodo.org/record/2553669. Now this need to be scaled up and tested more stringently.
Name | Size | |
---|---|---|
Optimisation of HTT-HAP40 purification using heparin affinity chromatography 20190129.docx
md5:570971e647502698275f9eca9403b7ce |
910.3 kB | Download |
Optimisation of HTT-HAP40 purification using heparin affinity chromatography 20190129.pdf
md5:aa4156d9a7ceae73535c0a72f3f4b540 |
508.3 kB | Download |
All versions | This version | |
---|---|---|
Views | 85 | 85 |
Downloads | 40 | 40 |
Data volume | 25.6 MB | 25.6 MB |
Unique views | 75 | 75 |
Unique downloads | 28 | 28 |