There is a newer version of this record available.

Dataset Open Access

Cloning and test expression of HTT fragment clones - 2019/02/28

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Lee, Matt

Dublin Core Export

<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="" xmlns:oai_dc="" xmlns:xsi="" xsi:schemaLocation="">
  <dc:creator>Harding, Rachel</dc:creator>
  <dc:creator>Hutchinson, Ashley</dc:creator>
  <dc:creator>Seitova, Alma</dc:creator>
  <dc:creator>Arrowsmith, Cheryl</dc:creator>
  <dc:creator>Lee, Matt</dc:creator>
  <dc:description>Project: High resolution structural analysis of purified HTT proteins

Experiment: Cloning and test expression of HTT fragment clones

Date completed:­ 2019/02/28

Rationale: I aim to complement our previous work on purifying stable regions of huntingtin by cloning further regions of huntingtin corresponding to discrete HEAT repeats for eukaryotic expression. Constructs will initially be screened by BVES in insect sf9 cells. High quality constructs will undergo extensive crystallization experiments and structure determination by X-ray crystallography using well established high-throughput and systematic protocols in place at the SGC. Any solved structures may allow generation of a pseudoatomic resolution (&lt;4 Å) model of the HTT protein using the cryoEM model as a guide. </dc:description>
  <dc:description>Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dc:description>
  <dc:subject>Protein expression</dc:subject>
  <dc:subject>Protein purification</dc:subject>
  <dc:subject>X-ray crystallography</dc:subject>
  <dc:title>Cloning and test expression of HTT fragment clones - 2019/02/28</dc:title>
All versions This version
Views 248145
Downloads 16681
Data volume 149.3 MB81.5 MB
Unique views 195122
Unique downloads 8252


Cite as