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Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells - 2019/01/28

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Lee, Matt


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{
  "description": "<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein samples</p>\n\n<p><strong>Experiment:&nbsp;</strong>Expression and purification of HTT<sup>79-3144&nbsp;</sup>with and without HAP40 from Sf9 Insect cells</p>\n\n<p><strong>Date completed:&shy;&nbsp;</strong>2019/01/28</p>\n\n<p><strong>Rationale:&nbsp;</strong>HTT lacking the unstructured region of exon1 (aa. 1-78 which are not resolved in the cryoEM model PDB ID 6EZ8) may serve as a useful control when determining exon1-dependent functionality of the HTT protein. Apo and HAP40-bound&nbsp;HTT<sup>79-3144&nbsp;</sup>will be produced from Sf9 insect cell expression system.&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Hutchinson, Ashley"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Seitova, Alma"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
      "affiliation": "CHDI Foundation", 
      "@type": "Person", 
      "name": "Lee, Matt"
    }
  ], 
  "url": "https://zenodo.org/record/2598804", 
  "datePublished": "2019-01-28", 
  "keywords": [
    "Huntingtin", 
    "Protein production", 
    "Huntington's disease", 
    "BVES production"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/c596393d-0f9d-4527-9168-172c81910068/Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells 20190128.docx", 
      "encodingFormat": "docx", 
      "@type": "DataDownload"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/c596393d-0f9d-4527-9168-172c81910068/Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells 20190128.pdf", 
      "encodingFormat": "pdf", 
      "@type": "DataDownload"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.2598804", 
  "@id": "https://doi.org/10.5281/zenodo.2598804", 
  "@type": "Dataset", 
  "name": "Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells - 2019/01/28"
}
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