Dataset Open Access

A time-course analysis using Differential Static Light Scattering (DSLS) of purified HTT1-3144 Q23 - 2019/01/28

Harding, Rachel; Arrowsmith, Cheryl; Lee, Matt


JSON-LD (schema.org) Export

{
  "description": "<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein samples</p>\n\n<p><strong>Experiment:&nbsp;</strong>A time-course analysis using Differential Static Light Scattering (DSLS) of purified HTT<sup>1-3144</sup>Q23&nbsp;</p>\n\n<p><strong>Date completed:&shy;&nbsp;</strong>2019/01/28</p>\n\n<p><strong>Rationale:&nbsp;</strong>Time and resources in the HD field have been primarily focussed on understanding HTT aggregation looking as caspase cleavage products spanning aa. 1-586 or exon 1 spanning aa. 1-90. However, we know that HTT protein purified in its apo form is able to self-associate into larger oligomeric species and that monomer, dimer and larger species are found following FLAG-affinity chromatography as determined by size-exclusion chromatography (SEC) and SEC-multi-angle light scattering (SEC-MALS). This experiment aimed to begin to investigate how HTT self-associates and aggregates over time in a range of different conditions.&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
      "affiliation": "CHDI Foundation", 
      "@type": "Person", 
      "name": "Lee, Matt"
    }
  ], 
  "url": "https://zenodo.org/record/2598762", 
  "datePublished": "2019-01-28", 
  "keywords": [
    "DSLS", 
    "Differential Static Light Scattering", 
    "Huntingtin", 
    "HTT", 
    "Huntington's disease"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/1bb45b9f-8e5b-4902-aede-2aa2a270c503/A time-course analysis using Differential Static Light Scattering (DSLS) of purified HTT1-3144 Q23 20190128.docx", 
      "encodingFormat": "docx", 
      "@type": "DataDownload"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/1bb45b9f-8e5b-4902-aede-2aa2a270c503/DSLS_20190128.xlsx", 
      "encodingFormat": "xlsx", 
      "@type": "DataDownload"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.2598762", 
  "@id": "https://doi.org/10.5281/zenodo.2598762", 
  "@type": "Dataset", 
  "name": "A time-course analysis using Differential Static Light Scattering (DSLS) of purified HTT1-3144 Q23 - 2019/01/28"
}
94
32
views
downloads
All versions This version
Views 9495
Downloads 3232
Data volume 8.5 MB8.5 MB
Unique views 7879
Unique downloads 2020

Share

Cite as