2582853
doi
10.3390/toxins11030137
oai:zenodo.org:2582853
user-eu
Development of an Antifungal and Antimycotoxigenic Device Containing Allyl Isothiocyanate for Silo Fumigation
Juan Manuel Quiles 1,†OrcID, Tiago de Melo Nazareth 1,2,†, Carlos Luz 1, Fernando Bittencourt Luciano 2OrcID, Jordi Mañes 1 and Giuseppe Meca 1,*
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
<p>The aims of this study were to evaluate the antifungal activity of the bioactive compound allyl isothiocyanate (AITC) against Aspergillus flavus (8111 ISPA) aflatoxins (AFs) producer and Penicillium verrucosum (D-01847 VTT) ochratoxin A (OTA) producer on corn, barley, and wheat. The experiments were carried out initially in a simulated silo system for laboratory scale composed of glass jars (1 L). Barley and wheat were contaminated with P. verrucosum and corn with A. flavus. The cereals were treated with a hydroxyethylcellulose gel disk to which 500 µL/L of AITC were added; the silo system was closed and incubated for 30 days at 21 °C. After that, simulated silos of 100 L capacity were used. Barley, wheat, and corn were contaminated under the same conditions as the previous trial and treated with disks with 5 mL of AITC, closed and incubated for 90 days at 21 °C. In both cases, the control test did not receive any antifungal treatment. The growth of the inoculated fungi and the reduction in the formation of AFs and OTA were determined. In the lab scale silo system, complete inhibition of fungal growth at 30 days has been observed. In corn, the reduction of aflatoxin B1 (AFB<sub>1</sub>) was 98.5%. In the 100 L plastic drums, a significant reduction in the growth of A. flavus was observed, as well as the OTA formation in wheat (99.5%) and barley (92.0%).</p>
Zenodo
2019-03-01
info:eu-repo/semantics/article
2582852
user-eu
award_title=Integrated and innovative key actions for mycotoxin management in the food and feed chain; award_number=678781; award_identifiers_scheme=url; award_identifiers_identifier=https://cordis.europa.eu/projects/678781; funder_id=00k4n6c32; funder_name=European Commission;
1579539035.764068
2415065
md5:871ece476eeb898d79e437b40c24a53c
https://zenodo.org/records/2582853/files/toxins-11-00137 MECA.pdf
public