2575486
doi
10.1016/j.plipres.2016.06.001
oai:zenodo.org:2575486
user-cosmos-h2020
Oroz-Guinea, Isabel
Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University
Brundiek, Henrieke
Enzymicals AG
Bornscheuer, Uwe T.
Enzymicals AG
Engineering and application of enzymes for lipid modification, an update
Zorn, Katja
Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
<p>This review first provides a brief introduction into the most important tools and strategies for protein engineering (i.e. directed evolution and rational protein design combined with high-throughput screening methods) followed by examples from literature, in which enzymes have been optimized for biocatalytic applications. This covers engineered lipases with altered fatty acid chain length selectivity, fatty acid specificity and improved performance in esterification reactions. Furthermore, recent achievements reported for phospholipases, lipoxygenases, P450 monooxygenases, decarboxylating enzymes, fatty acid hydratases and the use of enzymes in cascade reactions are treated. </p>
Zenodo
2016-06-11
info:eu-repo/semantics/article
2575485
user-cosmos-h2020
1579541433.938963
1400644
md5:a2c09eccecaecc9217f12ae65eb60edf
https://zenodo.org/records/2575486/files/PLR_2016_18_revised_Bornscheuer.pdf
public