Technical note Open Access

Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts

Joe Win; Emilie Chanclud; C. Sarai Reyes-Avila; Thorsten Langner; Tofazzal Islam; Sophien Kamoun


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="041" ind1=" " ind2=" ">
    <subfield code="a">eng</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">plant pathology</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">genomics</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">fungi</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">wheat</subfield>
  </datafield>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">rice</subfield>
  </datafield>
  <controlfield tag="005">20210311085140.0</controlfield>
  <controlfield tag="001">2564950</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">The Sainsbury Laboratory</subfield>
    <subfield code="0">(orcid)0000-0003-0381-7668</subfield>
    <subfield code="a">Emilie Chanclud</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">The Sainsbury Laboratory</subfield>
    <subfield code="0">(orcid)0000-0002-6970-8862</subfield>
    <subfield code="a">C. Sarai Reyes-Avila</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">The Sainsbury Laboratory</subfield>
    <subfield code="0">(orcid)0000-0002-3401-8888</subfield>
    <subfield code="a">Thorsten Langner</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Bangabandhu Sheikh Mujibur Rahman Agricultural University</subfield>
    <subfield code="0">(orcid)0000-0002-7613-0261</subfield>
    <subfield code="a">Tofazzal Islam</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">The Sainsbury Laboratory</subfield>
    <subfield code="0">(orcid)0000-0002-0290-0315</subfield>
    <subfield code="a">Sophien Kamoun</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">285368</subfield>
    <subfield code="z">md5:f514801501643fc66b58894b44bd3ffe</subfield>
    <subfield code="u">https://zenodo.org/record/2564950/files/Win_Zenodo - Nanopore sequencing of Mo with tables and figure 20190212_SUBMITTED.pdf</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2019-02-14</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="p">user-openriceblast</subfield>
    <subfield code="p">user-openwheatblast</subfield>
    <subfield code="o">oai:zenodo.org:2564950</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">The Sainsbury Laboratory</subfield>
    <subfield code="0">(orcid)0000-0002-9851-2404</subfield>
    <subfield code="a">Joe Win</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-openriceblast</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">user-openwheatblast</subfield>
  </datafield>
  <datafield tag="536" ind1=" " ind2=" ">
    <subfield code="c">743165</subfield>
    <subfield code="a">Retooling plant immunity for resistance to blast fungi</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;&lt;strong&gt;We report long-range sequencing of eight isolates of&amp;nbsp;&lt;em&gt;Magnaporthe oryzae&lt;/em&gt;(Syn.&amp;nbsp;&lt;em&gt;Pyricularia oryzae&lt;/em&gt;) from wheat, rice, foxtail millet and goosegrass using nanopore MinION. Our aim is to obtain chromosome-level genome assemblies that are freely available for public access to be scrutinized for genome rearrangements and structural variation.&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;em&gt;Magnaporthe oryzae&lt;/em&gt;(Syn.&amp;nbsp;&lt;em&gt;Pyricularia oryzae&lt;/em&gt;) is a notorious fungus known for causing blast disease on rice and wheat with devastating effect on grain yield.&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;host range includes several other cereal crops such as oat, finger millet and foxtail millet as well as wild grasses.&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;is found all over the world wherever warm temperature and high humidity are common. Although lineages of&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;tend to be adapted to a particular host, this pathogen can shift from one host to another when the conditions permit&amp;nbsp;(Couch&amp;nbsp;&lt;em&gt;et al.&lt;/em&gt;2005). We hypothesize that structural variation contributes to adaptation to new hosts and environmental conditions following, for example, the model proposed by&amp;nbsp;Chuma&amp;nbsp;&lt;em&gt;et al.&lt;/em&gt;(2011). To generate the genomics data that would enable such analyses, we sequenced eight isolates of&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;from four different hosts using long-range nanonpore MinION with the aim of providing chromosome-level genome assemblies that are freely available for public access.&amp;nbsp;&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="773" ind1=" " ind2=" ">
    <subfield code="n">doi</subfield>
    <subfield code="i">isVersionOf</subfield>
    <subfield code="a">10.5281/zenodo.2564949</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.5281/zenodo.2564950</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">publication</subfield>
    <subfield code="b">technicalnote</subfield>
  </datafield>
</record>
1,090
728
views
downloads
All versions This version
Views 1,0901,092
Downloads 728728
Data volume 207.7 MB207.7 MB
Unique views 1,0131,015
Unique downloads 674674

Share

Cite as