Technical note Open Access

Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts

Joe Win; Emilie Chanclud; C. Sarai Reyes-Avila; Thorsten Langner; Tofazzal Islam; Sophien Kamoun


JSON-LD (schema.org) Export

{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p><strong>We report long-range sequencing of eight isolates of&nbsp;<em>Magnaporthe oryzae</em>(Syn.&nbsp;<em>Pyricularia oryzae</em>) from wheat, rice, foxtail millet and goosegrass using nanopore MinION. Our aim is to obtain chromosome-level genome assemblies that are freely available for public access to be scrutinized for genome rearrangements and structural variation.</strong></p>\n\n<p><em>Magnaporthe oryzae</em>(Syn.&nbsp;<em>Pyricularia oryzae</em>) is a notorious fungus known for causing blast disease on rice and wheat with devastating effect on grain yield.&nbsp;<em>M. oryzae</em>host range includes several other cereal crops such as oat, finger millet and foxtail millet as well as wild grasses.&nbsp;<em>M. oryzae</em>is found all over the world wherever warm temperature and high humidity are common. Although lineages of&nbsp;<em>M. oryzae</em>tend to be adapted to a particular host, this pathogen can shift from one host to another when the conditions permit&nbsp;(Couch&nbsp;<em>et al.</em>2005). We hypothesize that structural variation contributes to adaptation to new hosts and environmental conditions following, for example, the model proposed by&nbsp;Chuma&nbsp;<em>et al.</em>(2011). To generate the genomics data that would enable such analyses, we sequenced eight isolates of&nbsp;<em>M. oryzae</em>from four different hosts using long-range nanonpore MinION with the aim of providing chromosome-level genome assemblies that are freely available for public access.&nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "The Sainsbury Laboratory", 
      "@id": "https://orcid.org/0000-0002-9851-2404", 
      "@type": "Person", 
      "name": "Joe Win"
    }, 
    {
      "affiliation": "The Sainsbury Laboratory", 
      "@id": "https://orcid.org/0000-0003-0381-7668", 
      "@type": "Person", 
      "name": "Emilie Chanclud"
    }, 
    {
      "affiliation": "The Sainsbury Laboratory", 
      "@id": "https://orcid.org/0000-0002-6970-8862", 
      "@type": "Person", 
      "name": "C. Sarai Reyes-Avila"
    }, 
    {
      "affiliation": "The Sainsbury Laboratory", 
      "@id": "https://orcid.org/0000-0002-3401-8888", 
      "@type": "Person", 
      "name": "Thorsten Langner"
    }, 
    {
      "affiliation": "Bangabandhu Sheikh Mujibur Rahman Agricultural University", 
      "@id": "https://orcid.org/0000-0002-7613-0261", 
      "@type": "Person", 
      "name": "Tofazzal Islam"
    }, 
    {
      "affiliation": "The Sainsbury Laboratory", 
      "@id": "https://orcid.org/0000-0002-0290-0315", 
      "@type": "Person", 
      "name": "Sophien Kamoun"
    }
  ], 
  "headline": "Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2019-02-14", 
  "url": "https://zenodo.org/record/2564950", 
  "keywords": [
    "plant pathology", 
    "genomics", 
    "fungi", 
    "wheat", 
    "rice"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.5281/zenodo.2564950", 
  "@id": "https://doi.org/10.5281/zenodo.2564950", 
  "@type": "ScholarlyArticle", 
  "name": "Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts"
}
1,090
728
views
downloads
All versions This version
Views 1,0901,092
Downloads 728728
Data volume 207.7 MB207.7 MB
Unique views 1,0131,015
Unique downloads 674674

Share

Cite as