Technical note Open Access

Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts

Joe Win; Emilie Chanclud; C. Sarai Reyes-Avila; Thorsten Langner; Tofazzal Islam; Sophien Kamoun


JSON Export

{
  "files": [
    {
      "links": {
        "self": "https://zenodo.org/api/files/72a8caaa-a722-4889-bcac-2b46c0d4b25f/Win_Zenodo%20-%20Nanopore%20sequencing%20of%20Mo%20with%20tables%20and%20figure%2020190212_SUBMITTED.pdf"
      }, 
      "checksum": "md5:f514801501643fc66b58894b44bd3ffe", 
      "bucket": "72a8caaa-a722-4889-bcac-2b46c0d4b25f", 
      "key": "Win_Zenodo - Nanopore sequencing of Mo with tables and figure 20190212_SUBMITTED.pdf", 
      "type": "pdf", 
      "size": 285368
    }
  ], 
  "owners": [
    54205
  ], 
  "doi": "10.5281/zenodo.2564950", 
  "stats": {
    "version_unique_downloads": 670.0, 
    "unique_views": 1013.0, 
    "views": 1090.0, 
    "version_views": 1088.0, 
    "unique_downloads": 670.0, 
    "version_unique_views": 1011.0, 
    "volume": 206606432.0, 
    "version_downloads": 724.0, 
    "downloads": 724.0, 
    "version_volume": 206606432.0
  }, 
  "links": {
    "doi": "https://doi.org/10.5281/zenodo.2564950", 
    "conceptdoi": "https://doi.org/10.5281/zenodo.2564949", 
    "bucket": "https://zenodo.org/api/files/72a8caaa-a722-4889-bcac-2b46c0d4b25f", 
    "conceptbadge": "https://zenodo.org/badge/doi/10.5281/zenodo.2564949.svg", 
    "html": "https://zenodo.org/record/2564950", 
    "latest_html": "https://zenodo.org/record/2564950", 
    "badge": "https://zenodo.org/badge/doi/10.5281/zenodo.2564950.svg", 
    "latest": "https://zenodo.org/api/records/2564950"
  }, 
  "conceptdoi": "10.5281/zenodo.2564949", 
  "created": "2019-02-14T11:48:10.973442+00:00", 
  "updated": "2021-03-11T08:51:40.072347+00:00", 
  "conceptrecid": "2564949", 
  "revision": 8, 
  "id": 2564950, 
  "metadata": {
    "access_right_category": "success", 
    "doi": "10.5281/zenodo.2564950", 
    "description": "<p><strong>We report long-range sequencing of eight isolates of&nbsp;<em>Magnaporthe oryzae</em>(Syn.&nbsp;<em>Pyricularia oryzae</em>) from wheat, rice, foxtail millet and goosegrass using nanopore MinION. Our aim is to obtain chromosome-level genome assemblies that are freely available for public access to be scrutinized for genome rearrangements and structural variation.</strong></p>\n\n<p><em>Magnaporthe oryzae</em>(Syn.&nbsp;<em>Pyricularia oryzae</em>) is a notorious fungus known for causing blast disease on rice and wheat with devastating effect on grain yield.&nbsp;<em>M. oryzae</em>host range includes several other cereal crops such as oat, finger millet and foxtail millet as well as wild grasses.&nbsp;<em>M. oryzae</em>is found all over the world wherever warm temperature and high humidity are common. Although lineages of&nbsp;<em>M. oryzae</em>tend to be adapted to a particular host, this pathogen can shift from one host to another when the conditions permit&nbsp;(Couch&nbsp;<em>et al.</em>2005). We hypothesize that structural variation contributes to adaptation to new hosts and environmental conditions following, for example, the model proposed by&nbsp;Chuma&nbsp;<em>et al.</em>(2011). To generate the genomics data that would enable such analyses, we sequenced eight isolates of&nbsp;<em>M. oryzae</em>from four different hosts using long-range nanonpore MinION with the aim of providing chromosome-level genome assemblies that are freely available for public access.&nbsp;</p>", 
    "language": "eng", 
    "title": "Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts", 
    "license": {
      "id": "CC-BY-4.0"
    }, 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "2564949"
          }, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "2564950"
          }
        }
      ]
    }, 
    "communities": [
      {
        "id": "openriceblast"
      }, 
      {
        "id": "openwheatblast"
      }
    ], 
    "grants": [
      {
        "code": "743165", 
        "links": {
          "self": "https://zenodo.org/api/grants/10.13039/501100000780::743165"
        }, 
        "title": "Retooling plant immunity for resistance to blast fungi", 
        "acronym": "BLASTOFF", 
        "program": "H2020", 
        "funder": {
          "doi": "10.13039/501100000780", 
          "acronyms": [], 
          "name": "European Commission", 
          "links": {
            "self": "https://zenodo.org/api/funders/10.13039/501100000780"
          }
        }
      }
    ], 
    "keywords": [
      "plant pathology", 
      "genomics", 
      "fungi", 
      "wheat", 
      "rice"
    ], 
    "publication_date": "2019-02-14", 
    "creators": [
      {
        "orcid": "0000-0002-9851-2404", 
        "affiliation": "The Sainsbury Laboratory", 
        "name": "Joe Win"
      }, 
      {
        "orcid": "0000-0003-0381-7668", 
        "affiliation": "The Sainsbury Laboratory", 
        "name": "Emilie Chanclud"
      }, 
      {
        "orcid": "0000-0002-6970-8862", 
        "affiliation": "The Sainsbury Laboratory", 
        "name": "C. Sarai Reyes-Avila"
      }, 
      {
        "orcid": "0000-0002-3401-8888", 
        "affiliation": "The Sainsbury Laboratory", 
        "name": "Thorsten Langner"
      }, 
      {
        "orcid": "0000-0002-7613-0261", 
        "affiliation": "Bangabandhu Sheikh Mujibur Rahman Agricultural University", 
        "name": "Tofazzal Islam"
      }, 
      {
        "orcid": "0000-0002-0290-0315", 
        "affiliation": "The Sainsbury Laboratory", 
        "name": "Sophien Kamoun"
      }
    ], 
    "access_right": "open", 
    "resource_type": {
      "subtype": "technicalnote", 
      "type": "publication", 
      "title": "Technical note"
    }, 
    "related_identifiers": [
      {
        "scheme": "doi", 
        "identifier": "10.5281/zenodo.2564949", 
        "relation": "isVersionOf"
      }
    ]
  }
}
1,088
724
views
downloads
All versions This version
Views 1,0881,090
Downloads 724724
Data volume 206.6 MB206.6 MB
Unique views 1,0111,013
Unique downloads 670670

Share

Cite as