Technical note Open Access

Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts

Joe Win; Emilie Chanclud; C. Sarai Reyes-Avila; Thorsten Langner; Tofazzal Islam; Sophien Kamoun


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  <identifier identifierType="DOI">10.5281/zenodo.2564950</identifier>
  <creators>
    <creator>
      <creatorName>Joe Win</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-9851-2404</nameIdentifier>
      <affiliation>The Sainsbury Laboratory</affiliation>
    </creator>
    <creator>
      <creatorName>Emilie Chanclud</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0003-0381-7668</nameIdentifier>
      <affiliation>The Sainsbury Laboratory</affiliation>
    </creator>
    <creator>
      <creatorName>C. Sarai Reyes-Avila</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-6970-8862</nameIdentifier>
      <affiliation>The Sainsbury Laboratory</affiliation>
    </creator>
    <creator>
      <creatorName>Thorsten Langner</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-3401-8888</nameIdentifier>
      <affiliation>The Sainsbury Laboratory</affiliation>
    </creator>
    <creator>
      <creatorName>Tofazzal Islam</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-7613-0261</nameIdentifier>
      <affiliation>Bangabandhu Sheikh Mujibur Rahman Agricultural University</affiliation>
    </creator>
    <creator>
      <creatorName>Sophien Kamoun</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-0290-0315</nameIdentifier>
      <affiliation>The Sainsbury Laboratory</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2019</publicationYear>
  <subjects>
    <subject>plant pathology</subject>
    <subject>genomics</subject>
    <subject>fungi</subject>
    <subject>wheat</subject>
    <subject>rice</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2019-02-14</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Technical note</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/2564950</alternateIdentifier>
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    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.2564949</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/openriceblast</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/openwheatblast</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;We report long-range sequencing of eight isolates of&amp;nbsp;&lt;em&gt;Magnaporthe oryzae&lt;/em&gt;(Syn.&amp;nbsp;&lt;em&gt;Pyricularia oryzae&lt;/em&gt;) from wheat, rice, foxtail millet and goosegrass using nanopore MinION. Our aim is to obtain chromosome-level genome assemblies that are freely available for public access to be scrutinized for genome rearrangements and structural variation.&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;em&gt;Magnaporthe oryzae&lt;/em&gt;(Syn.&amp;nbsp;&lt;em&gt;Pyricularia oryzae&lt;/em&gt;) is a notorious fungus known for causing blast disease on rice and wheat with devastating effect on grain yield.&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;host range includes several other cereal crops such as oat, finger millet and foxtail millet as well as wild grasses.&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;is found all over the world wherever warm temperature and high humidity are common. Although lineages of&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;tend to be adapted to a particular host, this pathogen can shift from one host to another when the conditions permit&amp;nbsp;(Couch&amp;nbsp;&lt;em&gt;et al.&lt;/em&gt;2005). We hypothesize that structural variation contributes to adaptation to new hosts and environmental conditions following, for example, the model proposed by&amp;nbsp;Chuma&amp;nbsp;&lt;em&gt;et al.&lt;/em&gt;(2011). To generate the genomics data that would enable such analyses, we sequenced eight isolates of&amp;nbsp;&lt;em&gt;M. oryzae&lt;/em&gt;from four different hosts using long-range nanonpore MinION with the aim of providing chromosome-level genome assemblies that are freely available for public access.&amp;nbsp;&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/743165/">743165</awardNumber>
      <awardTitle>Retooling plant immunity for resistance to blast fungi</awardTitle>
    </fundingReference>
  </fundingReferences>
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