Technical note Open Access

Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts

Joe Win; Emilie Chanclud; C. Sarai Reyes-Avila; Thorsten Langner; Tofazzal Islam; Sophien Kamoun


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.2564950", 
  "language": "eng", 
  "title": "Nanopore sequencing of genomic DNA from Magnaporthe oryzae isolates from different hosts", 
  "issued": {
    "date-parts": [
      [
        2019, 
        2, 
        14
      ]
    ]
  }, 
  "abstract": "<p><strong>We report long-range sequencing of eight isolates of&nbsp;<em>Magnaporthe oryzae</em>(Syn.&nbsp;<em>Pyricularia oryzae</em>) from wheat, rice, foxtail millet and goosegrass using nanopore MinION. Our aim is to obtain chromosome-level genome assemblies that are freely available for public access to be scrutinized for genome rearrangements and structural variation.</strong></p>\n\n<p><em>Magnaporthe oryzae</em>(Syn.&nbsp;<em>Pyricularia oryzae</em>) is a notorious fungus known for causing blast disease on rice and wheat with devastating effect on grain yield.&nbsp;<em>M. oryzae</em>host range includes several other cereal crops such as oat, finger millet and foxtail millet as well as wild grasses.&nbsp;<em>M. oryzae</em>is found all over the world wherever warm temperature and high humidity are common. Although lineages of&nbsp;<em>M. oryzae</em>tend to be adapted to a particular host, this pathogen can shift from one host to another when the conditions permit&nbsp;(Couch&nbsp;<em>et al.</em>2005). We hypothesize that structural variation contributes to adaptation to new hosts and environmental conditions following, for example, the model proposed by&nbsp;Chuma&nbsp;<em>et al.</em>(2011). To generate the genomics data that would enable such analyses, we sequenced eight isolates of&nbsp;<em>M. oryzae</em>from four different hosts using long-range nanonpore MinION with the aim of providing chromosome-level genome assemblies that are freely available for public access.&nbsp;</p>", 
  "author": [
    {
      "family": "Joe Win"
    }, 
    {
      "family": "Emilie Chanclud"
    }, 
    {
      "family": "C. Sarai Reyes-Avila"
    }, 
    {
      "family": "Thorsten Langner"
    }, 
    {
      "family": "Tofazzal Islam"
    }, 
    {
      "family": "Sophien Kamoun"
    }
  ], 
  "type": "article", 
  "id": "2564950"
}
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