2556608
doi
10.5281/zenodo.2556608
oai:zenodo.org:2556608
Elham Eslahi
Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran
Nazafarin Hatami-Mazinani
Department of Clinical Pharmacy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Elham Magharehabed
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
Mahsa Aliqanbari
Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran
Niloofar Ahmadi
Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran
Shabnam Naderifar
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Mona Mirgeloybayat
Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran
Hamed Hojatian
Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran
Mojdeh Naeima
Faculty of Pharmacy, First Moscow State Medical University, IM. Sechenov Moscow, Russia
Soha Sadeghi
Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran/ Department of Molecular genetics, Research Institute of NikanRoyesh Gene, Karaj, Iran
Study of the side effects of Docetaxel as chemotherapy medicine on changing the expression of genes of Enterococcus faecalis isolated from patients with breast cancer
Farnaz Rahbarzare
Department of Genetics and Molecular Biology, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia./ Department of microbial biotechnology, North Tehran Branch, Islamic Azad University, Tehran, Iran
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Breast cancer, Docetaxel, antibiotic resistance, Enterococcus faecalis
<p><strong>The present study strived to ascertain the side effects of chemotherapy on the pathogenic potential of <em>Enterococcus faecalis</em> (a natural microflora bacteria) isolated from patients with breast cancer. Participants included 400 female sufferers from breast cancer before and after the period of chemotherapy and 400 healthy people living with patients.After RNA extracted from the stool of all the participants, cDNA was constructed. Nineteen virulent genes (<em>vanB</em>, <em>vanA</em>, <em>VanC-3</em>,<em>VanC-2</em>, <em>VanC-1</em>, <em>aac(6′)-Ie-aph(2″)-Ia</em>, <em>Tet(L)</em>, <em>Erm(B)</em>, <em>gelE</em>, <em>esp</em>, <em>gyrA</em>,<em>slyA</em>, <em>cylA</em>,<em>cylB,cylM, asa1</em>, <em>aggA</em>, <em>efaA</em> and <em>enlA</em>) of <em>E. faecalis</em> were assessed by Real-Time qPCR. The findings revealed a statistically significant correlation between the expression level of fourteen virulence genes(<em>vanA</em>(p = 0.033)<em>, vanB</em>(p = 0.003)<em>, VanC-3</em>(p = 0.003)<em>, aac(6′)-Ie-aph(2″)-Ia</em>(p = 0.005)<em>, Erm(B)</em> (p = 0.008)<em>, gelE</em>(p = 0.002)<em>, esp</em>(p = 0.0005)<em>, gyrA</em>(p = 0.003)<em>, slyA</em>(p = 0.001)<em>, cylA</em>(p = 0.003)<em>, cylB</em>(p = 0.0002)<em>, asa1</em>(p = 0.003)<em>, aggA</em>(p = 0.003)<em>, efaA</em>(p = 0.003))in the group of the sufferers after chemotherapy and the other two groups (P<0.05).Additionally, the observation of patient group after chemotherapy showed an outstanding association between overexpression ofantibiotic resistance genes (<em>vanA, vanB, VanC-3, Erm(B), aac(6′)-Ie-aph(2″)-Ia</em>) and observation of the antibiotic resistance (vancomycin, erythromycin, gentamicin in respectively). Interestingly, while Docetaxel seems to be a suitable medicine to treat breast cancer, it may change the balance of mRNA expression of microflora. These could trigger infections during the cancer chemotherapy.</strong></p>
Zenodo
2019-02-04
info:eu-repo/semantics/article
2556607
1579539626.329315
162176
md5:2eed606805aea13e5087554b2465038f
https://zenodo.org/records/2556608/files/Rahbarzare et al.pdf
public
10.5281/zenodo.2556607
isVersionOf
doi