Journal article Open Access

Study of the side effects of Docetaxel as chemotherapy medicine on changing the expression of genes of Enterococcus faecalis isolated from patients with breast cancer

Farnaz Rahbarzare; Elham Eslahi; Nazafarin Hatami-Mazinani; Elham Magharehabed; Mahsa Aliqanbari; Niloofar Ahmadi; Shabnam Naderifar; Mona Mirgeloybayat; Hamed Hojatian; Mojdeh Naeima; Soha Sadeghi


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    <subfield code="a">Breast cancer, Docetaxel, antibiotic resistance, Enterococcus faecalis</subfield>
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    <subfield code="u">Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran</subfield>
    <subfield code="a">Elham Eslahi</subfield>
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    <subfield code="u">Department of Clinical Pharmacy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran</subfield>
    <subfield code="a">Nazafarin Hatami-Mazinani</subfield>
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    <subfield code="u">Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran</subfield>
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    <subfield code="u">Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran</subfield>
    <subfield code="a">Mahsa Aliqanbari</subfield>
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    <subfield code="a">Niloofar Ahmadi</subfield>
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    <subfield code="u">Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran</subfield>
    <subfield code="a">Shabnam Naderifar</subfield>
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    <subfield code="u">Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran</subfield>
    <subfield code="a">Mona Mirgeloybayat</subfield>
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    <subfield code="u">Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran</subfield>
    <subfield code="a">Hamed Hojatian</subfield>
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    <subfield code="u">Faculty of Pharmacy, First Moscow State Medical University, IM. Sechenov Moscow, Russia</subfield>
    <subfield code="a">Mojdeh Naeima</subfield>
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    <subfield code="u">Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran/ Department of Molecular genetics, Research Institute of NikanRoyesh Gene, Karaj, Iran</subfield>
    <subfield code="a">Soha Sadeghi</subfield>
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    <subfield code="c">2019-02-04</subfield>
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    <subfield code="a">Farnaz Rahbarzare</subfield>
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    <subfield code="a">Study of the side effects of Docetaxel as chemotherapy medicine on changing the expression of genes of Enterococcus faecalis isolated from patients with breast cancer</subfield>
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    <subfield code="a">&lt;p&gt;&lt;strong&gt;The present study strived to ascertain the side effects of chemotherapy on the pathogenic potential of &lt;em&gt;Enterococcus faecalis&lt;/em&gt; (a natural microflora bacteria) isolated from patients with breast cancer. Participants included 400 female sufferers from breast cancer before and after the period of chemotherapy and 400 healthy people living with patients.After RNA extracted from the stool of all the participants, cDNA was constructed. Nineteen virulent genes (&lt;em&gt;vanB&lt;/em&gt;, &lt;em&gt;vanA&lt;/em&gt;, &lt;em&gt;VanC-3&lt;/em&gt;,&lt;em&gt;VanC-2&lt;/em&gt;, &lt;em&gt;VanC-1&lt;/em&gt;, &lt;em&gt;aac(6&amp;prime;)-Ie-aph(2&amp;Prime;)-Ia&lt;/em&gt;, &lt;em&gt;Tet(L)&lt;/em&gt;, &lt;em&gt;Erm(B)&lt;/em&gt;, &lt;em&gt;gelE&lt;/em&gt;, &lt;em&gt;esp&lt;/em&gt;, &lt;em&gt;gyrA&lt;/em&gt;,&lt;em&gt;slyA&lt;/em&gt;, &lt;em&gt;cylA&lt;/em&gt;,&lt;em&gt;cylB,cylM, asa1&lt;/em&gt;, &lt;em&gt;aggA&lt;/em&gt;, &lt;em&gt;efaA&lt;/em&gt; and &lt;em&gt;enlA&lt;/em&gt;) of &lt;em&gt;E. faecalis&lt;/em&gt; were assessed by Real-Time qPCR. The findings revealed a statistically significant correlation between the expression level of fourteen virulence genes(&lt;em&gt;vanA&lt;/em&gt;(p = 0.033)&lt;em&gt;, vanB&lt;/em&gt;(p = 0.003)&lt;em&gt;, VanC-3&lt;/em&gt;(p = 0.003)&lt;em&gt;, aac(6&amp;prime;)-Ie-aph(2&amp;Prime;)-Ia&lt;/em&gt;(p = 0.005)&lt;em&gt;, Erm(B)&lt;/em&gt; (p = 0.008)&lt;em&gt;, gelE&lt;/em&gt;(p = 0.002)&lt;em&gt;, esp&lt;/em&gt;(p = 0.0005)&lt;em&gt;, gyrA&lt;/em&gt;(p = 0.003)&lt;em&gt;, slyA&lt;/em&gt;(p = 0.001)&lt;em&gt;, cylA&lt;/em&gt;(p = 0.003)&lt;em&gt;, cylB&lt;/em&gt;(p = 0.0002)&lt;em&gt;, asa1&lt;/em&gt;(p = 0.003)&lt;em&gt;, aggA&lt;/em&gt;(p = 0.003)&lt;em&gt;, efaA&lt;/em&gt;(p = 0.003))in the group of the sufferers after chemotherapy and the other two groups (P&amp;lt;0.05).Additionally, the observation of patient group after chemotherapy showed an outstanding association between overexpression ofantibiotic resistance genes (&lt;em&gt;vanA, vanB, VanC-3, Erm(B), aac(6&amp;prime;)-Ie-aph(2&amp;Prime;)-Ia&lt;/em&gt;) and observation of the antibiotic resistance (vancomycin, erythromycin, gentamicin in respectively). Interestingly, while Docetaxel seems to be a suitable medicine to treat breast cancer, it may change the balance of mRNA expression of microflora. These could trigger infections during the cancer chemotherapy.&lt;/strong&gt;&lt;/p&gt;</subfield>
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