Journal article Open Access

Study of the side effects of Docetaxel as chemotherapy medicine on changing the expression of genes of Enterococcus faecalis isolated from patients with breast cancer

Farnaz Rahbarzare; Elham Eslahi; Nazafarin Hatami-Mazinani; Elham Magharehabed; Mahsa Aliqanbari; Niloofar Ahmadi; Shabnam Naderifar; Mona Mirgeloybayat; Hamed Hojatian; Mojdeh Naeima; Soha Sadeghi


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  <identifier identifierType="DOI">10.5281/zenodo.2556608</identifier>
  <creators>
    <creator>
      <creatorName>Farnaz Rahbarzare</creatorName>
      <affiliation>Department of Genetics and Molecular Biology, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia./ Department of microbial biotechnology, North Tehran Branch, Islamic Azad University, Tehran, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Elham Eslahi</creatorName>
      <affiliation>Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Nazafarin Hatami-Mazinani</creatorName>
      <affiliation>Department of Clinical Pharmacy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Elham Magharehabed</creatorName>
      <affiliation>Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Mahsa Aliqanbari</creatorName>
      <affiliation>Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Niloofar Ahmadi</creatorName>
      <affiliation>Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Shabnam Naderifar</creatorName>
      <affiliation>Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Mona Mirgeloybayat</creatorName>
      <affiliation>Department of Microbiology, Research and Science branch, Islamic Azad University, Tehran, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Hamed Hojatian</creatorName>
      <affiliation>Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran</affiliation>
    </creator>
    <creator>
      <creatorName>Mojdeh Naeima</creatorName>
      <affiliation>Faculty of Pharmacy, First Moscow State Medical University, IM. Sechenov Moscow, Russia</affiliation>
    </creator>
    <creator>
      <creatorName>Soha Sadeghi</creatorName>
      <affiliation>Department of Cellular and Molecular Biology, NourDanesh Institute of Higher Education, Isfahan, Iran/ Department of Molecular genetics, Research Institute of NikanRoyesh Gene, Karaj, Iran</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Study of the side effects of Docetaxel as chemotherapy medicine on changing the expression of genes of Enterococcus faecalis isolated from patients with breast cancer</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2019</publicationYear>
  <subjects>
    <subject>Breast cancer, Docetaxel, antibiotic resistance, Enterococcus faecalis</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2019-02-04</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/2556608</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.2556607</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;The present study strived to ascertain the side effects of chemotherapy on the pathogenic potential of &lt;em&gt;Enterococcus faecalis&lt;/em&gt; (a natural microflora bacteria) isolated from patients with breast cancer. Participants included 400 female sufferers from breast cancer before and after the period of chemotherapy and 400 healthy people living with patients.After RNA extracted from the stool of all the participants, cDNA was constructed. Nineteen virulent genes (&lt;em&gt;vanB&lt;/em&gt;, &lt;em&gt;vanA&lt;/em&gt;, &lt;em&gt;VanC-3&lt;/em&gt;,&lt;em&gt;VanC-2&lt;/em&gt;, &lt;em&gt;VanC-1&lt;/em&gt;, &lt;em&gt;aac(6&amp;prime;)-Ie-aph(2&amp;Prime;)-Ia&lt;/em&gt;, &lt;em&gt;Tet(L)&lt;/em&gt;, &lt;em&gt;Erm(B)&lt;/em&gt;, &lt;em&gt;gelE&lt;/em&gt;, &lt;em&gt;esp&lt;/em&gt;, &lt;em&gt;gyrA&lt;/em&gt;,&lt;em&gt;slyA&lt;/em&gt;, &lt;em&gt;cylA&lt;/em&gt;,&lt;em&gt;cylB,cylM, asa1&lt;/em&gt;, &lt;em&gt;aggA&lt;/em&gt;, &lt;em&gt;efaA&lt;/em&gt; and &lt;em&gt;enlA&lt;/em&gt;) of &lt;em&gt;E. faecalis&lt;/em&gt; were assessed by Real-Time qPCR. The findings revealed a statistically significant correlation between the expression level of fourteen virulence genes(&lt;em&gt;vanA&lt;/em&gt;(p = 0.033)&lt;em&gt;, vanB&lt;/em&gt;(p = 0.003)&lt;em&gt;, VanC-3&lt;/em&gt;(p = 0.003)&lt;em&gt;, aac(6&amp;prime;)-Ie-aph(2&amp;Prime;)-Ia&lt;/em&gt;(p = 0.005)&lt;em&gt;, Erm(B)&lt;/em&gt; (p = 0.008)&lt;em&gt;, gelE&lt;/em&gt;(p = 0.002)&lt;em&gt;, esp&lt;/em&gt;(p = 0.0005)&lt;em&gt;, gyrA&lt;/em&gt;(p = 0.003)&lt;em&gt;, slyA&lt;/em&gt;(p = 0.001)&lt;em&gt;, cylA&lt;/em&gt;(p = 0.003)&lt;em&gt;, cylB&lt;/em&gt;(p = 0.0002)&lt;em&gt;, asa1&lt;/em&gt;(p = 0.003)&lt;em&gt;, aggA&lt;/em&gt;(p = 0.003)&lt;em&gt;, efaA&lt;/em&gt;(p = 0.003))in the group of the sufferers after chemotherapy and the other two groups (P&amp;lt;0.05).Additionally, the observation of patient group after chemotherapy showed an outstanding association between overexpression ofantibiotic resistance genes (&lt;em&gt;vanA, vanB, VanC-3, Erm(B), aac(6&amp;prime;)-Ie-aph(2&amp;Prime;)-Ia&lt;/em&gt;) and observation of the antibiotic resistance (vancomycin, erythromycin, gentamicin in respectively). Interestingly, while Docetaxel seems to be a suitable medicine to treat breast cancer, it may change the balance of mRNA expression of microflora. These could trigger infections during the cancer chemotherapy.&lt;/strong&gt;&lt;/p&gt;</description>
  </descriptions>
</resource>
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