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Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


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  <dc:creator>Harding, Rachel</dc:creator>
  <dc:creator>Hutchinson, Ashley</dc:creator>
  <dc:creator>Seitova, Alma</dc:creator>
  <dc:creator>Arrowsmith, Cheryl</dc:creator>
  <dc:creator>Edwards, Aled</dc:creator>
  <dc:date>2019-01-14</dc:date>
  <dc:description>Project - Huntingtin structure-function open lab notebook.  

Experiment - Optimisation of HTT-HAP40 purification using heparin affinity chromatography.

Aims - The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems will be carried out using heparin and FLAG affinity chromatography. </dc:description>
  <dc:identifier>https://zenodo.org/record/2553669</dc:identifier>
  <dc:identifier>10.5281/zenodo.2553669</dc:identifier>
  <dc:identifier>oai:zenodo.org:2553669</dc:identifier>
  <dc:relation>doi:10.5281/zenodo.2553668</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/labscribbles</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/openlabnotebooks</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>Huntingtin</dc:subject>
  <dc:subject>HAP40</dc:subject>
  <dc:subject>Protein purification</dc:subject>
  <dc:subject>Huntington's disease</dc:subject>
  <dc:title>Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
</oai_dc:dc>
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