Dataset Open Access

Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


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{
  "description": "<p><strong>Project</strong>&nbsp;- Huntingtin structure-function open lab notebook. &nbsp;</p>\n\n<p><strong>Experiment</strong>&nbsp;- Optimisation of HTT-HAP40 purification using heparin affinity chromatography.</p>\n\n<p><strong>Aims</strong> -&nbsp;The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin.&nbsp;To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems will be carried out using heparin and FLAG affinity chromatography.&nbsp;</p>", 
  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Hutchinson, Ashley"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Seitova, Alma"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Edwards, Aled"
    }
  ], 
  "url": "https://zenodo.org/record/2553669", 
  "datePublished": "2019-01-14", 
  "keywords": [
    "Huntingtin", 
    "HAP40", 
    "Protein purification", 
    "Huntington's disease"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/35a0177e-3ed7-4a04-b2a1-9de9b33ac562/Optimisation_HTT-HAP40_purification_Heparin_20190114.docx", 
      "@type": "DataDownload", 
      "fileFormat": "docx"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/35a0177e-3ed7-4a04-b2a1-9de9b33ac562/Optimisation_HTT-HAP40_purification_Heparin_20190114.pdf", 
      "@type": "DataDownload", 
      "fileFormat": "pdf"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.2553669", 
  "@id": "https://doi.org/10.5281/zenodo.2553669", 
  "@type": "Dataset", 
  "name": "Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)"
}
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