Atef Jaouani
Fatma Arous
Chadlia Hamdi
Souhir Kmiha
Nadia Khammassi
Amani Ayari
Mohamed Neifar
Tahar Mechichi
Atef Jaouani
2019-01-28
<p>This work describes the performance of a sequencing batch reactor (SBR) and the involvement of a novel reconstituted<br>
bacterial consortium in olive mill wastewater (OMW) treatment. The organic loading rate applied to the SBR was serially<br>
increased in terms of initial COD from 10 to 75 g L−1 to allow gradual acclimatization of activated sludge to high concentrations<br>
of toxic compounds in OMW. After the acclimatization period, up to 60% of the total COD content were effectively<br>
biodegraded from OMW at 75 g L−1 COD within 30 day hydraulic retention time. The diversity and community composition<br>
of cultivable bacteria participating in the aerobic process of treating OMW were further assessed. A total of 91 bacterial<br>
strains were isolated from the reactor and analyzed by amplification of the 16S-23S rRNA internal transcribed spacer (ITS)<br>
region and by 16S rRNA gene sequencing. The most abundant phylum was Firmicutes (57.1%) followed by Proteobacteria<br>
(35.2%) and Actinobacteria (7.7%). The use of the Biolog® Phenotype Microarray system to evaluate the ability of isolated<br>
strains to utilize OMW phenolic compounds is reported in this work for the first time. Interestingly, results showed that<br>
all species tested were able to utilize phenolics as sole carbon and energy sources. The removals of COD and phenolics<br>
from undiluted OMW by the reconstituted bacterial consortium were almost similar to those obtained by the acclimatized<br>
activated sludge, which suggest that cultivable bacteria play the major role in OMW biodegradation. Phytotoxicity assays<br>
using tomato seeds showed a significant improvement of seed germination values for treated OMW. Our overall results suggest<br>
that the novel developed bacterial consortium could be considered as a good prospect for phenolics-rich wastewaters<br>
bioremediation applications.</p>
https://doi.org/10.1007/s13205-018-1486-6
oai:zenodo.org:2551587
Zenodo
https://doi.org/10.5281/zenodo.2530273
https://zenodo.org/communities/eu
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
3 Biotech, 8, 481, (2019-01-28)
Acclimatized aerobic consortium
Biolog ® phenotyping
Olive mill wastewater
Phenolic compounds
phytotoxicity
Sequencing batch reactor
Treatment of olive mill wastewater through employing sequencing batch reactor: performance and microbial diversity assessment
info:eu-repo/semantics/article