10.1007/s13205-018-1486-6
https://zenodo.org/records/2551587
oai:zenodo.org:2551587
Fatma Arous
Fatma Arous
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, University of Tunis El Manar, 9, Rue Zouhair Essafi, 1007 Tunis, Tunisia
Chadlia Hamdi
Chadlia Hamdi
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, University of Tunis El Manar, 9, Rue Zouhair Essafi, 1007 Tunis, Tunisia
Souhir Kmiha
Souhir Kmiha
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, University of Tunis El Manar, 9, Rue Zouhair Essafi, 1007 Tunis, Tunisia
Nadia Khammassi
Nadia Khammassi
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, University of Tunis El Manar, 9, Rue Zouhair Essafi, 1007 Tunis, Tunisia
Amani Ayari
Amani Ayari
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, University of Tunis El Manar, 9, Rue Zouhair Essafi, 1007 Tunis, Tunisia
Mohamed Neifar
Mohamed Neifar
University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, Ariana, Tunisia
Tahar Mechichi
Tahar Mechichi
Laboratoire de Biochimie et de Genie Enzymatique des Lipases, ENIS, Route de Soukra, BPW 1173‑3038, Sfax, Tunisia
Atef Jaouani
Atef Jaouani
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, University of Tunis El Manar, 9, Rue Zouhair Essafi, 1007 Tunis, Tunisia
Treatment of olive mill wastewater through employing sequencing batch reactor: performance and microbial diversity assessment
Zenodo
2019
Acclimatized aerobic consortium
Biolog ® phenotyping
Olive mill wastewater
Phenolic compounds
phytotoxicity
Sequencing batch reactor
Atef Jaouani
Atef Jaouani
Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, University of Tunis El Manar, 9, Rue Zouhair Essafi, 1007 Tunis, Tunisia
2019-01-28
10.5281/zenodo.2530273
https://zenodo.org/communities/eu
Creative Commons Attribution 4.0 International
This work describes the performance of a sequencing batch reactor (SBR) and the involvement of a novel reconstituted
bacterial consortium in olive mill wastewater (OMW) treatment. The organic loading rate applied to the SBR was serially
increased in terms of initial COD from 10 to 75 g L−1 to allow gradual acclimatization of activated sludge to high concentrations
of toxic compounds in OMW. After the acclimatization period, up to 60% of the total COD content were effectively
biodegraded from OMW at 75 g L−1 COD within 30 day hydraulic retention time. The diversity and community composition
of cultivable bacteria participating in the aerobic process of treating OMW were further assessed. A total of 91 bacterial
strains were isolated from the reactor and analyzed by amplification of the 16S-23S rRNA internal transcribed spacer (ITS)
region and by 16S rRNA gene sequencing. The most abundant phylum was Firmicutes (57.1%) followed by Proteobacteria
(35.2%) and Actinobacteria (7.7%). The use of the Biolog® Phenotype Microarray system to evaluate the ability of isolated
strains to utilize OMW phenolic compounds is reported in this work for the first time. Interestingly, results showed that
all species tested were able to utilize phenolics as sole carbon and energy sources. The removals of COD and phenolics
from undiluted OMW by the reconstituted bacterial consortium were almost similar to those obtained by the acclimatized
activated sludge, which suggest that cultivable bacteria play the major role in OMW biodegradation. Phytotoxicity assays
using tomato seeds showed a significant improvement of seed germination values for treated OMW. Our overall results suggest
that the novel developed bacterial consortium could be considered as a good prospect for phenolics-rich wastewaters
bioremediation applications.
European Commission
10.13039/501100000780
688320
DevelopMent AnD application of integrated technological and management solutions FOR wasteWATER treatment and efficient reuse in agriculture tailored to the needs of Mediterranean African Countries