2541462
doi
10.5281/zenodo.2541462
oai:zenodo.org:2541462
user-drosophila
Stewart, James
Institute for Molecular and Cell Biology, A*STAR, Singapore 138673
Burhanudin, Safwan B.
Institute for Molecular and Cell Biology, A*STAR, Singapore 138673
Claridge-Chang, Adam
Institute for Molecular and Cell Biology, A*STAR, Singapore 138673 Department of Physiology, National University of Singapore, Singapore Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore Correspondence
Data for: Optogenetic olfactory behavior depends on illumination characteristics
Tumkaya, Tayfun
Institute for Molecular and Cell Biology, A*STAR, Singapore 138673 Department of Physiology, National University of Singapore, Singapore Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore Correspondence
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Drosophila
optogenetics
olfactory
behavior
<p><strong>Abstract</strong></p>
<p>Optogenetics has become an important tool to study behavior: it enables neuroscientists to infer causations by examining animal behavior after activating genetically circumscribed neurons with light. Light-induced activity is profoundly affected by illumination parameters used in experiments, such as intensity, duration, and frequency. How sensitive behavioral outcomes to light-dependent spike changes has not been extensively studied. Here, we investigated the hypothesis that light frequency would alter optogenetically induced behaviours. To test this, we activated olfactory receptor neurons (ORNs) in Drosophila by using either static- or pulsed-light stimuli. Static- and pulsed-light stimulations elicited distinct valence responses (attraction, aversion, neutral) to artificial activity in ORNs. Our results demonstrate the importance of light frequency for interpreting behavioral experiments accurately, and suggest that multiple light parameters should be tested before generalizing behavioral experiment results.</p>
Zenodo
2019-01-16
info:eu-repo/semantics/other
2541461
user-drosophila
1579591370.10183
2712411151
md5:e7a463a8bcd6e7c195b6fedbe545154b
https://zenodo.org/records/2541462/files/Data_and_Figures.rar
public
10.5281/zenodo.2541461
isVersionOf
doi