December 23, 2018 Journal article Open Access

Diana T, Dimitrios M, Eleni-Andrianna T, Tatiana T, Theodoros I, Maria D, Aspasia K, Kallirroi S, Panagiota N, Efstratios P, Maria KA, Maria K, Panayiotis P, Penelope K, Marie-Christine K

JSON Export

{
  "files": [
    {
      "links": {
        "self": "https://zenodo.org/api/files/c87246ba-fc64-4124-9b07-60affd4097e1/Triantafyllopoulou%20et%20al.pdf"
      }, 
      "checksum": "md5:e9ad9880c026fa2fb4654ef98999390f", 
      "bucket": "c87246ba-fc64-4124-9b07-60affd4097e1", 
      "key": "Triantafyllopoulou et al.pdf", 
      "type": "pdf", 
      "size": 138789
    }
  ], 
  "owners": [
    58503
  ], 
  "doi": "10.5281/zenodo.2536548", 
  "stats": {
    "version_unique_downloads": 6.0, 
    "unique_views": 6.0, 
    "views": 6.0, 
    "downloads": 7.0, 
    "unique_downloads": 6.0, 
    "version_unique_views": 6.0, 
    "volume": 971523.0, 
    "version_downloads": 7.0, 
    "version_views": 6.0, 
    "version_volume": 971523.0
  }, 
  "links": {
    "doi": "https://doi.org/10.5281/zenodo.2536548", 
    "conceptdoi": "https://doi.org/10.5281/zenodo.2536547", 
    "bucket": "https://zenodo.org/api/files/c87246ba-fc64-4124-9b07-60affd4097e1", 
    "conceptbadge": "https://zenodo.org/badge/doi/10.5281/zenodo.2536547.svg", 
    "html": "https://zenodo.org/record/2536548", 
    "latest_html": "https://zenodo.org/record/2536548", 
    "badge": "https://zenodo.org/badge/doi/10.5281/zenodo.2536548.svg", 
    "latest": "https://zenodo.org/api/records/2536548"
  }, 
  "conceptdoi": "10.5281/zenodo.2536547", 
  "created": "2019-01-09T20:02:21.033462+00:00", 
  "updated": "2019-01-10T01:32:36.978235+00:00", 
  "conceptrecid": "2536547", 
  "revision": 2, 
  "id": 2536548, 
  "metadata": {
    "access_right_category": "success", 
    "doi": "10.5281/zenodo.2536548", 
    "description": "<p>We evaluated the expression and intensity of IL-8 and OPG immunostaining in BM biopsies of MPN patients at diagnosis and correlated the results with clinical parameters. Seventy-six patients were studied, diagnosed with MPN from 1984 to 2012 with a median follow up period of 55 months. IL-8 and OPG immunostaining was performed on paraffin-embedded sections of formalin fixed BM biopsies, carried out at the time of diagnosis, using the two-step peroxidase conjugated polymer technique. Grade of positivity and intensity of IL-8 and OPG expression was scored according to a 0 to 3 scale. Statistical analysis was performed conventionally, using the SPSS version 22.0 package, and p values &lt;0.05 were considered significant. High intensity of IL-8 expression in megakaryocytes correlated, in the whole cohort, with neutrophilia (p=0.005) and high haemoglobin (p=0.001). High intensity of OPG expression in megakaryocytes correlated high haemoglobin (p=0.017) and a favorable karyotype (p=0.01). Most importantly, low OPG intensity in megakaryocytes correlated with poor survival (p=0.026) in the whole patient series. In conclusion, our most important finding is that high intensity of OPG expression in megakaryocytes correlated with increased survival and a favorable karyotype in patients with MPN, a finding that has not been reported yet.</p>", 
    "license": {
      "id": "CC-BY-4.0"
    }, 
    "title": "Bone marrow osteoprotegerin (OPG) and interleukin-8 (IL-8) immunostaining in patients with Ph-negative myeloproliferative neoplasms (MPN): Clinical correlations", 
    "journal": {
      "volume": "6", 
      "issue": "12", 
      "pages": "432-439", 
      "title": "Merit Research Journal of Medicine and Medical Sciences"
    }, 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "2536547"
          }, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "2536548"
          }
        }
      ]
    }, 
    "keywords": [
      "Myeloproliferative Neoplasms (MPN), Polycythemia Vera (PV), Essential Thrombocytosis (ET), Primary Myelofibrosis (MF), Osteoprotegerin (OPG), Interleukin-8 (IL-8)"
    ], 
    "publication_date": "2018-12-23", 
    "creators": [
      {
        "affiliation": "1Haematology Section of First Department of Propaedeutic Internal Medicine, Laikon Hospital, National and Kapodistrian University of Athens' Medical School, Athens, Greece;  2Department of Pathology, National and Kapodistrian University of Athens' Medical School, Athens, Greece; 3Department of Haematology, National and Kapodistrian University of Athens' Medical School, Athens, Greece", 
        "name": "Diana T, Dimitrios M, Eleni-Andrianna T, Tatiana T, Theodoros I, Maria D,        Aspasia K, Kallirroi S, Panagiota N, Efstratios P, Maria KA, Maria K, Panayiotis P, Penelope K, Marie-Christine K"
      }
    ], 
    "access_right": "open", 
    "resource_type": {
      "subtype": "article", 
      "type": "publication", 
      "title": "Journal article"
    }, 
    "related_identifiers": [
      {
        "scheme": "doi", 
        "relation": "isVersionOf", 
        "identifier": "10.5281/zenodo.2536547"
      }
    ]
  }
}