Journal article Open Access
Diana T, Dimitrios M, Eleni-Andrianna T, Tatiana T, Theodoros I, Maria D, Aspasia K, Kallirroi S, Panagiota N, Efstratios P, Maria KA, Maria K, Panayiotis P, Penelope K, Marie-Christine K
<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd"> <identifier identifierType="DOI">10.5281/zenodo.2536548</identifier> <creators> <creator> <creatorName>Diana T, Dimitrios M, Eleni-Andrianna T, Tatiana T, Theodoros I, Maria D, Aspasia K, Kallirroi S, Panagiota N, Efstratios P, Maria KA, Maria K, Panayiotis P, Penelope K, Marie-Christine K</creatorName> <affiliation>1Haematology Section of First Department of Propaedeutic Internal Medicine, Laikon Hospital, National and Kapodistrian University of Athens' Medical School, Athens, Greece; 2Department of Pathology, National and Kapodistrian University of Athens' Medical School, Athens, Greece; 3Department of Haematology, National and Kapodistrian University of Athens' Medical School, Athens, Greece</affiliation> </creator> </creators> <titles> <title>Bone marrow osteoprotegerin (OPG) and interleukin-8 (IL-8) immunostaining in patients with Ph-negative myeloproliferative neoplasms (MPN): Clinical correlations</title> </titles> <publisher>Zenodo</publisher> <publicationYear>2018</publicationYear> <subjects> <subject>Myeloproliferative Neoplasms (MPN), Polycythemia Vera (PV), Essential Thrombocytosis (ET), Primary Myelofibrosis (MF), Osteoprotegerin (OPG), Interleukin-8 (IL-8)</subject> </subjects> <dates> <date dateType="Issued">2018-12-23</date> </dates> <resourceType resourceTypeGeneral="Text">Journal article</resourceType> <alternateIdentifiers> <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/2536548</alternateIdentifier> </alternateIdentifiers> <relatedIdentifiers> <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.2536547</relatedIdentifier> </relatedIdentifiers> <rightsList> <rights rightsURI="http://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights> <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights> </rightsList> <descriptions> <description descriptionType="Abstract"><p>We evaluated the expression and intensity of IL-8 and OPG immunostaining in BM biopsies of MPN patients at diagnosis and correlated the results with clinical parameters. Seventy-six patients were studied, diagnosed with MPN from 1984 to 2012 with a median follow up period of 55 months. IL-8 and OPG immunostaining was performed on paraffin-embedded sections of formalin fixed BM biopsies, carried out at the time of diagnosis, using the two-step peroxidase conjugated polymer technique. Grade of positivity and intensity of IL-8 and OPG expression was scored according to a 0 to 3 scale. Statistical analysis was performed conventionally, using the SPSS version 22.0 package, and p values &lt;0.05 were considered significant. High intensity of IL-8 expression in megakaryocytes correlated, in the whole cohort, with neutrophilia (p=0.005) and high haemoglobin (p=0.001). High intensity of OPG expression in megakaryocytes correlated high haemoglobin (p=0.017) and a favorable karyotype (p=0.01). Most importantly, low OPG intensity in megakaryocytes correlated with poor survival (p=0.026) in the whole patient series. In conclusion, our most important finding is that high intensity of OPG expression in megakaryocytes correlated with increased survival and a favorable karyotype in patients with MPN, a finding that has not been reported yet.</p></description> </descriptions> </resource>
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