PREPARATION, CHARACTERIZATION AND DETERMINATION OF ANTIOXIDANT ACTIVITY OF ANTERDHUM PADHATI MASHI (APM) AND BAHIRDHUM PADHATI MASHI (BPM) OF COCOS NUCIFERA HUSK

Objective: To prepare and characterise AnterdhumpadhatiMashi (APM) and BahirdhumPadhatiMashi (BPM) of Cocosnucifera husk. Methods: The APM and BPM were prepared as per theAyurvedicliterature The physicochemical characterization was performed by Atomic Absorption Spectroscopy, Fourier transform infrared spectroscopy, Powder X-ray diffraction. Antioxidant activity was determined by DPPH assay, H2O2 Scavenging Activity, Reducing Power ability. Results: In DSC the region of 80 to 1200C weakening of hydrogen bonds occur with the loss of physically bounded water. This is evident from the weakening of the endothermic peak in both the mashi. An endotherm at 2100C in BPM may be assigned to the condensation product of lignin.PXRD study of confirmed the crystalline form of potassium as potassium chloride sylvite.Peaks at 3647 cm-1 and 3456 cm-1 are assigned to Stretching vibration mode of the OH-, 2889 cm-1, 2857cm-1 indicatesthe aliphatic C-H stretching. The bands between 1480 cm-1 and 1300 cm-1 may be due to the OH bending vibration which indicates the presence of the phenolic group. The bands around 2400 cm-1 denote the presence of C=O stretching indicating that lignin might be rich of methoxy-O-CH3, C-O-C stretching and C=C stretching (aromatic ring) containing compounds.RESULT: BPM is the powerful free radical scavenger. Conclusion: BPM can be use as an antioxidant agent.The findings of the present research work may be helpful for formulation and standardization of APM and BPM in routine analysis.